Tubulin Beta Class V Monoclonal antibody
Tubulin Beta Class V Monoclonal Antibody for IF, IHC, WB, ELISA
Cat no : 66362-1-Ig
Validation Data Gallery
|Positive WB detected in
|HeLa cells, human testis tissue, NCCIT cell, NIH/3T3 cells
|Positive IHC detected in
|human lung cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Western Blot (WB)
|WB : 1:500-1:2000
|IHC : 1:50-1:500
|IF : 1:50-1:500
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
66362-1-Ig targets Tubulin Beta Class V in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, Canine samples.
|human, mouse, Canine
|Host / Isotype
|Mouse / IgG1
|tubulin, beta 6
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Protein G purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Tubulin beta class V, encoded by TUBB6, is an isotype of beta tubulin, the major component of microtubule. It is widely expressed in many tissues. In normal tissues, tubulin beta class V is focally expressed in ciliary structure, while in cancer tissues it exhibited a diffuse staining pattern. The alterations of its expression in tumors suggest tubulin beta class V may be used as a biomarker for some solid tumors.
|Product Specific Protocols
|WB protocol for Tubulin Beta Class V antibody 66362-1-Ig
|IHC protocol for Tubulin Beta Class V antibody 66362-1-Ig
|IF protocol for Tubulin Beta Class V antibody 66362-1-Ig
|Click here to view our Standard Protocols
LncRNA MDRL Mitigates Atherosclerosis through miR-361/SQSTM1/NLRP3 Signaling
NPY stimulates cholesterol synthesis acutely by activating the SREBP2-HMGCR pathway through the Y1 and Y5 receptors in murine hepatocytes.
Alpha- and beta-tubulin isotypes are differentially expressed during brain development.
Proteomics and transcriptomics profiling reveals distinct aspects of kidney stone related genes in calculi rats