Tubulin Beta Class V Monoclonal antibody

Tubulin Beta Class V Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1


human, mouse, Canine and More (1)







Cat no : 66362-1-Ig


HsT1601, TUBB 5, TUBB6, Tubulin beta 6 chain, Tubulin beta class V, tubulin, beta 6

Tested Applications

Positive WB detected inHeLa cells, human testis tissue, NCCIT cell, NIH/3T3 cells
Positive IHC detected inhuman lung cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMDCK cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66362-1-Ig targets Tubulin Beta Class V in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, Canine samples.

Tested Reactivity human, mouse, Canine
Cited Reactivitymouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name tubulin, beta 6
Calculated Molecular Weight 50 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberNM_032525
Gene Symbol TUBB6
Gene ID (NCBI) 84617
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Tubulin beta class V, encoded by TUBB6, is an isotype of beta tubulin, the major component of microtubule. It is widely expressed in many tissues. In normal tissues, tubulin beta class V is focally expressed in ciliary structure, while in cancer tissues it exhibited a diffuse staining pattern. The alterations of its expression in tumors suggest tubulin beta class V may be used as a biomarker for some solid tumors.


Product Specific Protocols
WB protocol for Tubulin Beta Class V antibody 66362-1-IgDownload protocol
IHC protocol for Tubulin Beta Class V antibody 66362-1-IgDownload protocol
IF protocol for Tubulin Beta Class V antibody 66362-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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