Tubulin Beta Class V Monoclonal antibody
Tubulin Beta Class V Monoclonal Antibody for IF, IHC, WB, ELISA
Cat no : 66362-1-Ig
|Positive WB detected in||HeLa cells, human testis tissue, NCCIT cell, NIH/3T3 cells|
|Positive IHC detected in||human lung cancer tissue, human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||MDCK cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
66362-1-Ig targets Tubulin Beta Class V in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, dog samples.
|Tested Reactivity||human, mouse, dog|
|Cited Reactivity||mouse, rat|
|Host / Isotype||Mouse / IgG1|
|Full Name||tubulin, beta 6|
|Calculated molecular weight||50 kDa|
|Observed molecular weight||50 kDa|
|GenBank accession number||NM_032525|
|Gene ID (NCBI)||84617|
|Purification Method||Protein G purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Tubulin beta class V, encoded by TUBB6, is an isotype of beta tubulin, the major component of microtubule. It is widely expressed in many tissues. In normal tissues, tubulin beta class V is focally expressed in ciliary structure, while in cancer tissues it exhibited a diffuse staining pattern. The alterations of its expression in tumors suggest tubulin beta class V may be used as a biomarker for some solid tumors.
|Product Specific Protocols|
|WB protocol for Tubulin Beta Class V antibody 66362-1-Ig||Download protocol|
|IHC protocol for Tubulin Beta Class V antibody 66362-1-Ig||Download protocol|
|IF protocol for Tubulin Beta Class V antibody 66362-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
Alpha- and beta-tubulin isotypes are differentially expressed during brain development.
NPY stimulates cholesterol synthesis acutely by activating the SREBP2-HMGCR pathway through the Y1 and Y5 receptors in murine hepatocytes.