ChromoTek TurboGFP-Trap Agarose beads

Validation Data Gallery

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  Immunoprecipitation of TurboGFP with Turbo-GFP-Trap

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  Immunoprecipitation of TurboGFP. I: Input, FT: Flow-through, B: Bound. Coomassie blue stained gel and Western blot.

TurboGFP-Trap Agarose is an affinity bead for immunoprecipitation (IP) of TurboGFP fusion proteins It comprises an anti-TurboGFP VHH/ Nanobody coupled to agarose beads.

Cat No. tbta

Publications(15)

Reviews (2)

Specificity

TurboGFP, CopGFP, maxGFP

Applications

IP, CoIP

Type

Nanobody

Conjugate

Agarose

Synonyms

GFP-Trap, GFP-Trap Agarose, GFP-Trap Agarose beads, tbta, tbta-10, tbta-100, tbta-20, tbta-200, tbta-400, TurboGFP, TurboGFP tag, TurboGFP-tag, TurboGFP-Trap, TurboGFP-Trap Agarose beads

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Product Information

TurboGFP-Trap Agarose is an affinity bead for immunoprecipitation (IP) of TurboGFP fusion proteins It comprises an anti-TurboGFP VHH/ Nanobody coupled to agarose beads.

• Description: Immunoprecipitation of TurboGFP-fusion proteins and their interacting factors with anti-TurboGFP Nanobody conjugated to beads.

  • Fast, reliable & efficient one-step immunoprecipitation

  • Ready-to-use

  • No heavy & light antibody chains

  • Stable under harsh washing conditions

  • Suitable for downstream mass spec analysis

  • Works in samples from: mammals, plants, bacteria, yeast, insects etc.

• Applications: IP, CoIP
• Specificity/Target: TurboGFP, CopGFP, maxGFP, maxFP-Green ; For the complete list, please click here: Fluorescent protein specificity table
• Binding capacity: 7.5-10 μg of recombinant TurboGFP per 25 μL bead slurry
• Conjugate: Agarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads)
• Elution buffer: SDS sample buffer; 0.1 mM citrate acid pH 3.0
• Wash buffer compatibility: 6 M urea, 2 M NaCl, 10 mM DTT, 2 % Nonidet P40 Substitute, 1 % Triton X-100, 0.2 % SDS
• Type: Nanobody
• Class: Recombinant
• Host: Alpaca
• Affinity (KD): Dissociation constant K_D of 0.5 nM
• Compatibility with mass spectrometry: The TurboGFP-Trap is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry
• RRID: AB_2827596
• Storage Buffer: 20% ethanol
• Storage Condition: Shipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze!
• Size: 25ul/reactions (eg：20rxns=500ul slurry)

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Publications

Application	Title
	Mol Cancer; Tumour-intrinsic PDL1 signals regulate the Chk2 DNA damage response in cancer cells and mediate resistance to Chk1 inhibitors; Authors - Clare E Murray
IP	Nat Immunol; IRAP(+) endosomes restrict TLR9 activation and signaling.; Authors - Joel Babdor
IP	Nat Cell Biol; FERARI is required for Rab11-dependent endocytic recycling.; Authors - Jachen A Solinger
IP	Mol Metab; The pseudokinase TRIB3 controls adipocyte lipid homeostasis and proliferation in vitro and in vivo; Authors - Miguel Hernández-Quiles
IP	Cancers (Basel); Comprehensive Profiling of Mammalian Tribbles Interactomes Implicates TRIB3 in Gene Repression.; Authors - Miguel Hernández-Quiles
IP	Front Cell Dev Biol; Modulation of Increased mGluR1 Signaling by RGS8 Protects Purkinje Cells From Dendritic Reduction and Could Be a Common Mechanism in Diverse Forms of Spinocerebellar Ataxia.; Authors - Qin-Wei Wu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Sergio (Verified Customer) (01-08-2024)

It worked. The protocol provided is easy to follow.

• Applications: Immunoprecipitation

FH

Tobias (Verified Customer) (10-25-2022)

excellent efficiency and yield

• Applications: Immunoprecipitation