ChromoTek TurboGFP-Trap Magnetic Agarose

TurboGFP-Trap Magnetic Agarose is an affinity reagent for immunoprecipitation (IP) of TurboGFP fusion proteins.

Specificity

TurboGFP, CopGFP, maxGFP

Applications

IP, CoIP

Conjugate

Magnetic agarose

Type

Nanobody

Publications

2

Reviews (1)

Cat no : tbtma

Synonyms

TurboGFP-Trap, TurboGFP, CopGFP, maxGFP,maxFP-Green, green fluorescent protein CopGFP, TurboGFP tag

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Validation Data Gallery

• 

  Coomassie blue stained gel and Western blot.

• 

  TurboGFP-Trap Magnetic Agarose for pull-down of TurboGFP fusion proteins: Coomassie and Western Blot Input (I), non-bound (FT), and bound (B).

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Product Information

TurboGFP-Trap Magnetic Agarose is an affinity reagent for immunoprecipitation (IP) of TurboGFP fusion proteins.

• Description: Immunoprecipitation of TurboGFP-fusion proteins and their interacting factors with anti-TurboGFP Nanobody conjugated to magnetic agarose beads.

  • Fast, reliable & efficient one-step immunoprecipitation

  • Ready-to-use

  • No heavy & light antibody chains

  • Stable under harsh washing conditions

  • Suitable for downstream mass spec analysis

  • Works in samples from: mammals, plants, bacteria, yeast, insects etc.

• Applications: IP, CoIP
• Specificity/Target: TurboGFP, CopGFP, maxGFP, maxFP-Green ; For the complete list, please click here: Fluorescent protein specificity table
• Binding capacity: 7.5-10 μg of recombinant TurboGFP per 25 μL bead slurry
• Conjugate: Magnetic agarose beads; bead size: ~40 µm (cross-linked 6 % magnetic agarose beads)
• Elution buffer: SDS sample buffer; 0.1 mM citrate acid pH 3.0
• Wash buffer compatibility: 6 M urea, 2 M NaCl, 10 mM DTT, 2 % Nonidet P40 Substitute, 1 % Triton X-100, 0.2 % SDS
• Type: Nanobody
• Class: Recombinant
• Host: Alpaca
• Affinity (KD): Dissociation constant K_D of 0.5 nM
• Compatibility with mass spectrometry: The TurboGFP-Trap is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry
• RRID: AB_2827597
• Storage Buffer: 20% ethanol
• Storage Condition: Shipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze!

Other Formats

TurboGFP Trap Agarose kit

TurboGFP Trap Agarose

TurboGFP-Trap Magnetic Agarose kit

Publications

Application	Title
CoIP	Mol Biol Cell; RUSC2 and WDR47 oppositely regulate kinesin-1-dependent distribution of ATG9A to the cell periphery.; Authors - Carlos M Guardia
IP	Front Immunol; SIRT2-PFKP interaction dysregulates phagocytosis in macrophages with acute ethanol-exposure; Authors - Anugraha Gandhirajan

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Peter (Verified Customer) (10-24-2022)

Excellent IP system for a GFP variant with limited reagents available

• Applications: Immunoprecipitation
• Cell Tissue Type: U2OS