Cat no : tbtma
TurboGFP-Trap Magnetic Agarose is an affinity reagent for immunoprecipitation (IP) of TurboGFP fusion proteins.
|Description||Immunoprecipitation of TurboGFP-fusion proteins and their interacting factors with anti-TurboGFP Nanobody conjugated to magnetic agarose beads.
• Fast, reliable & efficient one-step immunoprecipitation
• No heavy & light antibody chains
• Stable under harsh washing conditions
• Suitable for downstream mass spec analysis
• Works in samples from: mammals, plants, bacteria, yeast, insects etc.
|Specificity/Target||TurboGFP, CopGFP, maxGFP, maxFP-Green |
For the complete list, please click here: Fluorescent protein specificity table
|Binding capacity||7.5-10 μg of recombinant TurboGFP per 25 μL bead slurry|
|Conjugate||Magnetic agarose beads; bead size: ~40 µm (cross-linked 6 % magnetic agarose beads)|
|Elution buffer||SDS sample buffer|
0.1 mM citrate acid pH 3.0
|Wash buffer compatibility||6 M urea, 2 M NaCl, 10 mM DTT, 2 % Nonidet P40 Substitute, 1 % Triton X-100, 0.2 % SDS|
|Affinity (KD)||Dissociation constant KD of 0.5 nM|
|Compatibility with mass spectrometry||The TurboGFP-Trap is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry|
|Storage Buffer||20% ethanol|
|Storage Condition||Shipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze!|
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Peter (Verified Customer) (10-24-2022)
Excellent IP system for a GFP variant with limited reagents available