Validation Data Gallery
Product Information
TurboGFP-Trap Magnetic Agarose is an affinity reagent for immunoprecipitation (IP) of TurboGFP fusion proteins.
| Description | Immunoprecipitation of TurboGFP-fusion proteins and their interacting factors with anti-TurboGFP Nanobody conjugated to magnetic agarose beads.
• Fast, reliable & efficient one-step immunoprecipitation • Ready-to-use • No heavy & light antibody chains • Stable under harsh washing conditions • Suitable for downstream mass spec analysis • Works in samples from: mammals, plants, bacteria, yeast, insects etc. |
| Applications | IP, CoIP |
| Specificity/Target | TurboGFP, CopGFP, maxGFP, maxFP-Green For the complete list, please click here: Fluorescent protein specificity table |
| Binding capacity | 7.5-10 μg of recombinant TurboGFP per 25 μL bead slurry |
| Conjugate | Magnetic agarose beads; bead size: ~40 µm (cross-linked 6 % magnetic agarose beads) |
| Elution buffer | SDS sample buffer 0.1 mM citrate acid pH 3.0 |
| Wash buffer compatibility | 6 M urea, 2 M NaCl, 10 mM DTT, 2 % Nonidet P40 Substitute, 1 % Triton X-100, 0.2 % SDS |
| Type | Nanobody |
| Class | Recombinant |
| Host | Alpaca |
| Affinity (KD) | Dissociation constant KD of 0.5 nM |
| Compatibility with mass spectrometry | The TurboGFP-Trap is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry |
| RRID | AB_2827597 |
| Storage Buffer | 20% ethanol |
| Storage Condition | Shipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze! |
| Size | 25ul/reactions (eg：20rxns=500ul slurry) |
Documentation
| SDS |
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| tbtma_SDS_TurboGFP-Trap Magnetic Agarose (EN) |
| Datasheet |
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| Protocol TurboGFP-Trap Magnetic Agarose (PDF) |
| Troubleshooting |
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| Troubleshooting guide immunoprecipitation (PDF) |
| Specificity |
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| Fluorescent protein specificity table (PDF) |
Publications
| Application | Title |
|---|---|
Mol Biol Cell RUSC2 and WDR47 oppositely regulate kinesin-1-dependent distribution of ATG9A to the cell periphery. | |
Front Immunol SIRT2-PFKP interaction dysregulates phagocytosis in macrophages with acute ethanol-exposure | |
Development CHD4 and SMYD1 repress common transcriptional programs in the developing heart |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Xi (Verified Customer) (05-01-2024) | Worked well for HEK293 cells transfected with turboGFP-tagged constructs
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FH Veronic (Verified Customer) (04-26-2024) | IPed Turbo-GFP fusion protein overexpressed in HEK293 cells. Great signal both in WB and MS Interactome analysis.
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FH Peter (Verified Customer) (10-24-2022) | Excellent IP system for a GFP variant with limited reagents available
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