ChromoTek TurboGFP-Trap Agarose beads, kit

TurboGFP-Trap Agarose is an affinity bead for immunoprecipitation (IP) of TurboGFP fusion proteins It comprises an anti-TurboGFP VHH/ Nanobody coupled to agarose beads.

Specificity

TurboGFP, CopGFP, maxGFP

Applications

IP, CoIP, ELISA

Conjugate

Agarose

Cat no : tbtak

Synonyms

Agarose bead, kit, GFP-Trap, GFP-Trap Agarose beads, tbtak, tbtak-20, TurboGFP, TurboGFP tag, TurboGFP-tag, TurboGFP-Trap Agarose beads



Product Information

TurboGFP-Trap Agarose is an affinity bead for immunoprecipitation (IP) of TurboGFP fusion proteins It comprises an anti-TurboGFP VHH/ Nanobody coupled to agarose beads.

ApplicationsIP, CoIP, ELISA
Clonality VHH
HostAlpaca
AffinityDissociation constant KD of 0.5 nM
Specificity/TargetTurboGFP, CopGFP, Pontellina plumata, maxGFP, maxFP-Green
Coupled VHHRecombinant, monoclonal anti-TurboGFP single domain antibody (sdAb) fragment
ConjugateAgarose
Beads~ 90 µm (cross-linked 4% agarose beads)
Elution bufferSDS sample buffer
0.1 mM citrate acid pH 3.0
Storage Buffer20% ethanol
Storage Condition Upon receipt store at +4°C. Do not freeze!

Kit components

ComponentDescription
TurboGFP-Trap Agarose beads20 reactions (500 µL)
Lysis bufferOptimized for cytoplasmatic proteins and mammalian cell lysis
RIPA bufferOptimized for nuclear/chromatin proteins and mammalian cell lysis
Wash buffer Removal of unwanted proteins, peptides, etc.
Dilution bufferDilution of cell lysate
Elution bufferFor acidic elution