ChromoTek TurboGFP-Trap Agarose beads, kit

TurboGFP-Trap Agarose is an affinity bead for immunoprecipitation (IP) of TurboGFP fusion proteins It comprises an anti-TurboGFP VHH/ Nanobody coupled to agarose beads.

Specificity

TurboGFP, CopGFP, maxGFP

Applications

IP, CoIP

Type

Nanobody

Conjugate

Agarose

Cat no : tbtak

Synonyms

Agarose bead, kit, GFP-Trap, GFP-Trap Agarose beads, tbtak, tbtak-20, TurboGFP, TurboGFP tag, TurboGFP-tag, TurboGFP-Trap Agarose beads



Product Information

TurboGFP-Trap Agarose is an affinity bead for immunoprecipitation (IP) of TurboGFP fusion proteins It comprises an anti-TurboGFP VHH/ Nanobody coupled to agarose beads.

DescriptionThe TurboGFP-Trap Agarose Kit contains TurboGFP-Trap Agarose, lysis, wash, and elution buffers for efficient immunoprecipitation of TurboGFP-fusion proteins and their interacting factors.

• Fast, reliable & efficient one-step immunoprecipitation

• Ready-to-use

• No heavy & light antibody chains on the SDS-PAGE

• Stable under harsh washing conditions

• Suitable for downstream mass spec analysis

ApplicationsIP, CoIP
Specificity/TargetTurboGFP, CopGFP, maxGFP, maxFP-Green
For the complete list, please click here: Fluorescent protein specificity table
Binding capacity7.5-10 μg of recombinant TurboGFP per 25 μL bead slurry
ConjugateAgarose beads; bead size: ~ 90 µm (cross-linked 4 % agarose beads)
Elution bufferSDS sample buffer
0.1 mM citrate acid pH 3.0
Wash buffer compatibility6 M urea, 2 M NaCl, 10 mM DTT, 2 % Nonidet P40 Substitute, 1 % Triton X-100, 0.2 % SDS
Type Nanobody
ClassRecombinant
HostAlpaca
Affinity (KD) Dissociation constant KD of 0.5 nM
Compatibility with mass spectrometryThe TurboGFP-Trap is optimized for on-bead digestion. For the application note, please click here: On-bead digest protocol for mass spectrometry
RRIDAB_2827596
Storage Buffer20% ethanol
Storage ConditionShipped at ambient temperature. Upon receipt store at 4°C. Stable for one year. Do not freeze!

Kit components

ComponentDescription
TurboGFP-Trap Agarose beads20 reactions (500 µL)
Lysis bufferOptimized for cytoplasmatic proteins and mammalian cell lysis
RIPA bufferOptimized for nuclear/chromatin proteins and mammalian cell lysis
Wash buffer Removal of unwanted proteins, peptides, etc.
Dilution bufferDilution of cell lysate
Elution bufferFor acidic elution

Publications

ApplicationTitle
IP

Cell Death Discov

Epigenetic mechanisms of Strip2 in differentiation of pluripotent stem cells

Authors - Sureshkumar Perumal Srinivasan