ChromoTek TurboGFP-Trap Magnetic Agarose, kit

TurboGFP-Trap Magnetic Agarose is an affinity reagent for immunoprecipitation (IP) of TurboGFP fusion proteins. It consists of an anti-TurboGFP VHH/ Nanobody that is coupled to magnetic agarose beads.

Specificity

TurboGFP, CopGFP, maxGFP

Applications

IP

Conjugate

Magnetic Agarose

Cat no : tbtmak

Synonyms

TurboGFP-Trap, TurboGFP, CopGFP, maxGFP,maxFP-Green, green fluorescent protein CopGFP, TurboGFP tag



Product Information

TurboGFP-Trap Magnetic Agarose is an affinity reagent for immunoprecipitation (IP) of TurboGFP fusion proteins. It consists of an anti-TurboGFP VHH/ Nanobody that is coupled to magnetic agarose beads.

ApplicationsIP
Clonality VHH
HostAlpaca
AffinityDissociation constant KD of 0.5 nM
Specificity/TargetTurboGFP, CopGFP, Pontellina plumata, maxGFP, maxFP-Green
Coupled VHHRecombinant, monoclonal anti-TurboGFP single domain antibody (sdAb) fragment
ConjugateMagnetic Agarose
Beads~ 40 µm
Binding capacity7.5-10 µg of recombinant TurboGFP per 25 µL bead slurry
Elution bufferSDS sample buffer
0.1 mM citrate acid pH 3.0
Storage Buffer20% ethanol
Storage ConditionShipped at ambient temperature. Upon receipt store at 4°C. Stable for one year.

Kit components

ComponentDescription
TurboGFP-Trap Magnetic Agarose20 reactions (500 µL)
Lysis bufferOptimized for cytoplasmatic proteins and mammalian cell lysis
RIPA bufferOptimized for nuclear/chromatin proteins and mammalian cell lysis
Wash buffer Removal of unwanted proteins, peptides, etc.
Dilution bufferDilution of cell lysate
Elution bufferFor acidic elution