U2AF35 Polyclonal antibody

• Featured Product
• KD/KO Validated

U2AF35 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

11

Cat no : 10334-1-AP

Synonyms

U2AF1, U2AFBP, RNU2AF1, RN, FP793

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Validation Data Gallery

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  WB analysis of HeLa using 10334-1-AP

  WB result of U2AF35 antibody (10334-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-U2AF35 transfected HeLa cells.

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  WB analysis of COLO 320 using 10334-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 10334-1-AP (U2AF35 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10334-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10334-1-AP (U2AF35 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 10334-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10334-1-AP (U2AF35 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human prostate cancer using 10334-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10334-1-AP (U2AF35 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of mouse small intestine using 10334-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 10334-1-AP (U2AF35 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: COLO 320 cells, HeLa cells
• Positive IHC detected in: mouse small intestine tissue, human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 10 publications below

Product Information

10334-1-AP targets U2AF35 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: U2AF35 fusion protein Ag0399
• Full Name: U2 small nuclear RNA auxiliary factor 1
• Calculated Molecular Weight: 28 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC001923
• Gene Symbol: U2AF1
• Gene ID (NCBI): 7307
• RRID: AB_2211314
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

U2 auxiliary factor (U2AF), comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. U2 (RNU2) small nuclear RNA auxiliary factor 1 (U2AF1,synonyms: RN, FP793, U2AF35, U2AFBP, RNU2AF1)is the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers. The U2AF1 gene is localized to chromosome 21q22.3, which is the critical region for three diseases, progressive myoclonus epilepsy, autoimmune polyglandular disease type 1, and one form of bipolar affective disorder.

Publications

Species	Application	Title
human	WB	Mol Cell; U2AF35(S34F) Promotes Transformation by Directing Aberrant ATG7 Pre-mRNA 3' End Formation.; Authors - Sung Mi Park
human	WB	Nucleic Acids Res; PUF60-activated exons uncover altered 3' splice-site selection by germline missense mutations in a single RRM.; Authors - Jana Královicová
human	WB	Cancer Res; PHF5A Epigenetically Inhibits Apoptosis to Promote Breast Cancer Progression.; Authors - Yi-Zi Zheng
human		Nucleic Acids Res; Identification of U2AF(35)-dependent exons by RNA-Seq reveals a link between 3' splice-site organization and activity of U2AF-related proteins.; Authors - Jana Kralovicova
human	WB	Int J Mol Sci; Splicing Enhancers at Intron-Exon Borders Participate in Acceptor Splice Sites Recognition.; Authors - Tatiana Kováčová
human	WB	J Biol Chem; Splicing factor SPF30 bridges an interaction between the prespliceosome protein U2AF35 and tri-small nuclear ribonucleoprotein protein hPrp3.; Authors - Little John T JT