CoraLite®594-conjugated U2AF35 Monoclonal antibody

U2AF35 Monoclonal Antibody for FC (Intra), IF

Host / Isotype

Mouse / IgG2c

Reactivity

human

Applications

IF, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2B7F11

Cat no : CL594-60289

Synonyms

DKFZp313J1712, FP793, RN, RNU2AF1, U2AF1, U2AF35, U2AFBP

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Validation Data Gallery

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  IF Staining of HepG2 using CL594-60289

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CL594-60289 (U2AF35 antibody) at dilution of 1:100.

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  FC experiment of Ramos using CL594-60289

  1X10^6 Ramos cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human U2AF35 (CL594-60289, Clone:2B7F11) (red), or 0.8 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive IF detected in: HepG2 cells
• Positive FC detected in: Ramos cells

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-60289 targets U2AF35 in IF, FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2c
• Class: Monoclonal
• Type: Antibody
• Immunogen: U2AF35 fusion protein Ag0399
• Full Name: U2 small nuclear RNA auxiliary factor 1
• Calculated Molecular Weight: 28 kDa
• Observed Molecular Weight: 35-40 kDa
• GenBank Accession Number: BC001923
• Gene Symbol: U2AF1
• Gene ID (NCBI): 7307
• RRID: AB_2883455
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

U2 auxiliary factor (U2AF), comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. U2 (RNU2) small nuclear RNA auxiliary factor 1 (U2AF1,synonyms: RN, FP793, U2AF35, U2AFBP, RNU2AF1)is the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers. The U2AF1 gene is localized to chromosome 21q22.3, which is the critical region for three diseases, progressive myoclonus epilepsy, autoimmune polyglandular disease type 1, and one form of bipolar affective disorder. The calculated molecular weight of U2AF35 is 28 kDa, but the modified U2AF35 protein is about 35-40 kDa.