UBA5 Polyclonal antibody

• Home
• Products
• Primary Antibodies
• UBA5 Polyclonal antibody (12093-1-AP)

• Featured Product
• KD/KO Validated

UBA5 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 12093-1-AP Copied!

Publications (17)

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Synonyms

ThiFP1, UBE1DC1, Ubiquitin activating enzyme 5, Ubiquitin-activating enzyme 5, Ubiquitin-activating enzyme E1 domain-containing protein 1

• 

  WB analysis using 12093-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12093-1-AP (UBA5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HEK-293 using 12093-1-AP

  WB result of UBA5 antibody (12093-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-UBA5 transfected HEK-293 cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HeLa using 12093-1-AP

  IP result of anti-UBA5 (IP:12093-1-AP, 4ug; Detection:12093-1-AP 1:1000) with HeLa cells lysate 1800ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon cancer using 12093-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12093-1-AP (UBA5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon cancer using 12093-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12093-1-AP (UBA5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HepG2 using 12093-1-AP

  Immunofluorescent analysis of HepG2 cells, using UBA5 antibody 12093-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 12093-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using UBA5 antibody (12093-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of HeLa using 12093-1-AP

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug UBA5 Polyclonal antibody (12093-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Able AI

What's the best dilution for UBA5 in ?

Chat with Able

---

Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, Jurkat cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells, HeLa cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12093-1-AP targets UBA5 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2734

  Product name: Recombinant human UBA5 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 105-404 aa of BC009737

  Sequence: YDKVELANMNRLFFQPHQAGLSKVQAAEHTLRNINPDVLFEVHNYNITTVENFQHFMDRISNGGLEEGKPVDLVLSCVDNFEARMTINTACNELGQTWMESGVSENAVSGHIQLIIPGESACFACAPPLVVAANIDEKTLKREGVCAASLPTTMGVVAGILVQNVLKFLLNFGTVSFYLGYNAMQDFFPTMSMKPNPQCDDRNCRKQQEEYKKKVAALPKQEVIQEEEEIIHEDNEWGIELVSEVSEEELKNFSGPVPDLPEGITVAYTIPKKQEDSVTELTVEDSGESLEDLMAKMKNM

• Full Name: ubiquitin-like modifier activating enzyme 5
• Calculated Molecular Weight: 404 aa, 45 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC009737
• Gene Symbol: UBA5
• Gene ID (NCBI): 79876
• RRID: AB_2211743
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9GZZ9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UBA5(Ubiquitin-like modifier-activating enzyme 5) is also named as UBE1DC1,ThiFP1.It has been detected highest UBE1DC1 expression in spleen, high expression in most other tissues examined, and low expression in colon, prostate, brain, and ovary by RT-PCR analysis(PMID:16328888).

Publications

What published studies show

The UBA5 Polyclonal antibody (Cat# 12093-1-AP) has 17 citations published in journals including Nature, Nature Communications, Molecular Cell, Science Advances, EMBO Journal, Molecular Neurodegenerations, American Journal of Human Genetics, Cell Reports, and others. Research fields span the UFMylation pathway, Alzheimer's disease, breast cancer progression, antiviral mechanisms, anti-tumor immunity, GPCR trafficking, DNA replication, mitochondrial autophagy, lipid droplet biogenesis, and cardiac homeostasis.

Automatically generated from the 17 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human		Nature; UFM1 E3 ligase promotes recycling of 60S ribosomal subunits from the ER; Authors - Paul A DaRosa
human	WB	Mol Neurodegener; The UFMylation pathway is impaired in Alzheimer's disease; Authors - Tingxiang Yan
human	WB	Nat Commun; A genus-wide interaction atlas across NS4B orthologues identifies a conserved role for UFMylation in orthoflavivirus replication.; Authors - Sreejith Rajasekharan ; KO Validated
human	WB	Nat Commun; The UFL1-AKT positive feedback loop promotes breast cancer progression by enhancing lipid synthesis.; Authors - Fei Meng
		Mol Cell; The UFSP2-ODR4 complex spatially confines and dynamically controls UFM1 deconjugation to safeguard neuronal proteostasis.; Authors - Gaoxin Mao
human	WB	Mol Cell; UFL1 ablation in T cells suppresses PD-1 UFMylation to enhance anti-tumor immunity; Authors - Chuan He ; KD Validated