UBAP2 Recombinant monoclonal antibody

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  WB analysis of HeLa using 83885-2-RR

  WB result of UBAP2 antibody (83885-2-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-UBAP2 transfected HeLa cells.

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  WB analysis using 83885-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83885-2-RR (UBAP2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 83885-2-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using UBAP2 antibody (83885-2-RR, Clone: 240890F6 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of U-2 OS using 83885-2-RR

  1x10^6 U-2 OS were intracellularly stained with 0.25 ug UBAP2 Recombinant antibody (83885-2-RR, Clone:240890F6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

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  Affinity and Kinetic Characterization of 83885-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83885-2-RR against Human UBAP2 were performed. The affinity constant is 0.59 nM.

• Featured Product
• KD/KO Validated

UBAP2 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Cat No. 83885-2-RR

Clone No.240890F6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

240890F6, bA176F3.5, KIAA1491, UBAP 2, ubiquitin associated protein 2

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Tested Applications

• Positive WB detected in: HeLa cells, HCT 116 cells, HEK-293T cells, A549 cells, Jurkat cells, U2OS cells
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: U-2 OS

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83885-2-RR targets UBAP2 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag30572

  Product name: Recombinant human UBAP2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 395-515 aa of NM_018449

  Sequence: QQNSTSHPTTTTSWDLKPPTSQSSVLSHLDFKSQPEPSPVLSQLSQRQQHQSQAVTVPPPGLESFPSQAKLRESTPGDSPSTVNKLLQLPSTTIENISVSVHQPQPKHIKLAKRRIPPASK

• Full Name: ubiquitin associated protein 2
• Calculated Molecular Weight: 117 kDa
• Observed Molecular Weight: 140 kDa
• GenBank Accession Number: NM_018449
• Gene Symbol: UBAP2
• Gene ID (NCBI): 55833
• RRID: AB_3671467
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q5T6F2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.