|Positive WB detected in||HEK-293T cells, L02 cells, SMMC-7721 cells, Ramos cells|
|Positive IP detected in||L02 cells|
|Positive IHC detected in||mouse ovary tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||L02 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
14520-1-AP targets UBC12 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||UBC12 fusion protein Ag5984|
|Full Name||ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast)|
|Calculated molecular weight||21 kDa|
|Observed molecular weight||21 kDa|
|GenBank accession number||BC058924|
|Gene ID (NCBI)||9040|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
|Product Specific Protocols|
|WB protocol for UBC12 antibody 14520-1-AP||Download protocol|
|IHC protocol for UBC12 antibody 14520-1-AP||Download protocol|
|IF protocol for UBC12 antibody 14520-1-AP||Download protocol|
|IP protocol for UBC12 antibody 14520-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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A potent small-molecule inhibitor of the DCN1-UBC12 interaction that selectively blocks cullin 3 neddylation.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Maria (Verified Customer) (02-06-2018)
I tested a free sample of this antibody.No non-specific bands.Load 40ug of protein for a well (15 well comb)I wish I could use a higher dilution of the antibody.