Validation Data Gallery
|Positive WB detected in
|HEK-293T cells, L02 cells, SMMC-7721 cells, HEK-293 cells, Ramos cells
|Positive IP detected in
|Positive IHC detected in
|mouse ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Western Blot (WB)
|WB : 1:500-1:2000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:50-1:500
|IF : 1:20-1:200
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
14520-1-AP targets UBC12 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|human, mouse, rat
|Host / Isotype
|Rabbit / IgG
|UBC12 fusion protein Ag5984
|ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast)
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
|Product Specific Protocols
|WB protocol for UBC12 antibody 14520-1-AP
|IHC protocol for UBC12 antibody 14520-1-AP
|IF protocol for UBC12 antibody 14520-1-AP
|IP protocol for UBC12 antibody 14520-1-AP
|Click here to view our Standard Protocols
A potent small-molecule inhibitor of the DCN1-UBC12 interaction that selectively blocks cullin 3 neddylation.
Genome-wide CRISPR screen identifies noncanonical NF-κB signaling as a regulator of density-dependent proliferation.
Cancer Biol Med
CUL4 E3 ligase regulates the proliferation and apoptosis of lung squamous cell carcinoma and small cell lung carcinoma.
Exp Cell Res
NPRL2 reduces the niraparib sensitivity of castration-resistant prostate cancer via interacting with UBE2M and enhancing neddylation.
Biochem Biophys Res Commun
Disruption of protein neddylation with MLN4924 attenuates paclitaxel-induced apoptosis and microtubule polymerization in ovarian cancer cells.
Biochem Cell Biol
Evaluation of HZX-960, a novel DCN1-UBC12 interaction inhibitor, as a potential antifibrotic compound for liver fibrosis.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Maria (Verified Customer) (02-06-2018)
I tested a free sample of this antibody.No non-specific bands.Load 40ug of protein for a well (15 well comb)I wish I could use a higher dilution of the antibody.