• Featured Product
  • KD/KO Validated

UBC12 Polyclonal antibody

UBC12 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14520-1-AP

Synonyms

hUbc12, NEDD8 carrier protein, NEDD8 conjugating enzyme Ubc12, NEDD8 protein ligase, UBC RS2, UBC12, UBE2M



Tested Applications

Positive WB detected inHEK-293T cells, L02 cells, SMMC-7721 cells, HEK-293 cells, Ramos cells
Positive IP detected inL02 cells
Positive IHC detected inmouse ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inL02 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

14520-1-AP targets UBC12 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UBC12 fusion protein Ag5984
Full Name ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast)
Calculated molecular weight 21 kDa
Observed molecular weight 21 kDa
GenBank accession numberBC058924
Gene symbol UBE2M
Gene ID (NCBI) 9040
RRIDAB_2878063
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for UBC12 antibody 14520-1-APDownload protocol
IHC protocol for UBC12 antibody 14520-1-APDownload protocol
IF protocol for UBC12 antibody 14520-1-APDownload protocol
IP protocol for UBC12 antibody 14520-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Nat Commun

A potent small-molecule inhibitor of the DCN1-UBC12 interaction that selectively blocks cullin 3 neddylation.

Authors - Haibin Zhou
humanWB

Elife

Genome-wide CRISPR screen identifies noncanonical NF-κB signaling as a regulator of density-dependent proliferation.

Authors - Maria Fomicheva
  • KO Validated
humanWB

Cancer Biol Med

CUL4 E3 ligase regulates the proliferation and apoptosis of lung squamous cell carcinoma and small cell lung carcinoma.

Authors - Ting Li
humanIHC,WB, IF

Exp Cell Res

NPRL2 reduces the niraparib sensitivity of castration-resistant prostate cancer via interacting with UBE2M and enhancing neddylation.

Authors - Xin Zhao
humanWB

Biochem Biophys Res Commun

Disruption of protein neddylation with MLN4924 attenuates paclitaxel-induced apoptosis and microtubule polymerization in ovarian cancer cells.

Authors - Xiaoling Hong
humanWB

Biochem Cell Biol

Evaluation of HZX-960, a novel DCN1-UBC12 interaction inhibitor, as a potential antifibrotic compound for liver fibrosis.

Authors - Wenjuan Zhou

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Maria (Verified Customer) (02-06-2018)

I tested a free sample of this antibody.No non-specific bands.Load 40ug of protein for a well (15 well comb)I wish I could use a higher dilution of the antibody.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:250-1:500
  • Cell Tissue Type: EpH4 mammary gland cell line
UBC12 Antibody Western Blot, validation (1:250-1:500 dilution) in EpH4 mammary gland cell line (Cat no:14520-1-AP)