UBC12 Monoclonal antibody

UBC12 Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

1H8D2

Cat no : 67482-1-Ig

Synonyms

hUbc12, NEDD8 carrier protein, NEDD8 conjugating enzyme Ubc12, NEDD8 protein ligase, UBC RS2, UBC12, UBE2M



Tested Applications

Positive WB detected inJurkat cells, HeLa cells, HEK-293 cells, HepG2 cells, L02 cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

67482-1-Ig targets UBC12 in WB, IHC,ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen UBC12 fusion protein Ag5984
Full Name ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast)
Calculated molecular weight 21 kDa
Observed molecular weight 21 kDa
GenBank accession numberBC058924
Gene symbol UBE2M
Gene ID (NCBI) 9040
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for UBC12 antibody 67482-1-IgDownload protocol
IHC protocol for UBC12 antibody 67482-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols