|Positive WB detected in||mouse thymus tissue|
|Positive IP detected in||mouse heart tissue|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:300 for WB|
|Sample-dependent, check data in validation data gallery|
51018-2-AP targets UBC9 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse, pig|
|Host / Isotype||Rabbit / IgG|
|Full Name||ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast)|
|Calculated molecular weight||18 kDa|
|Observed molecular weight||18 kDa|
|GenBank accession number||BC051289|
|Gene ID (NCBI)||7329|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Aliquoting is unnecessary for -20oC storage.|
UBC9 is also named as UBE2I, UBCE9 and belongs to the ubiquitin-conjugating enzyme family. It is a homologue of the E2 ubiquitin conjugating enzyme and participates in the covalent linking of SUMO-1 molecule to the target protein. This protein is present at a high level in spleen and lung. Moderate level of UBC9 is detected in kidney and liver. Low amount of UBC9 is observed in brain, whereas the 18 kDa band of UBC9 is barely visible or absent in heart and skeletal muscle. In heart and muscle extracts the UBC9 antibodies recognizes a 38 kDa protein band,but this band is not visible in extracts of other rat tissues(PMID:14739995).
Mechanism of Adenovirus E4-ORF3-Mediated SUMO Modifications.
IGSF11 is required for pericentric heterochromatin dissociation during meiotic diplotene.
J Biol Chem
Pathogenic mutations in the Valosin-containing protein/p97(VCP) N-domain inhibit the SUMOylation of VCP and lead to impaired stress response.
Foot-and-Mouth Disease Virus Counteracts on Internal Ribosome Entry Site Suppression by G3BP1 and Inhibits G3BP1-Mediated Stress Granule Assembly via Post-Translational Mechanisms.
PDPK1 regulates autophagosome biogenesis by binding to PIK3C3.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Marie (Verified Customer) (03-05-2020)
Not working on rat cells/tissus. Working on Humain ( GFP-Ubc9 human overexpression)