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  • KD/KO Validated

UBC9 Polyclonal antibody

UBC9 Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP,ELISA

Conjugate

Unconjugated

Cat no : 51018-2-AP

Synonyms

C358B7.1, E2,UBE2I, P18, SUMO conjugating enzyme UBC9, SUMO protein ligase, UBC9, UBCE9, UBE2I, Ubiquitin carrier protein 9, Ubiquitin carrier protein I, Ubiquitin protein ligase I



Tested Applications

Positive WB detected inmouse thymus tissue
Positive IP detected inmouse heart tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:300 for WB
Sample-dependent, check data in validation data gallery

Product Information

51018-2-AP targets UBC9 in WB, IP,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast)
Calculated molecular weight 18 kDa
Observed molecular weight 18 kDa
GenBank accession numberBC051289
Gene symbol UBE2I
Gene ID (NCBI) 7329
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

UBC9 is also named as UBE2I, UBCE9 and belongs to the ubiquitin-conjugating enzyme family. It is a homologue of the E2 ubiquitin conjugating enzyme and participates in the covalent linking of SUMO-1 molecule to the target protein. This protein is present at a high level in spleen and lung. Moderate level of UBC9 is detected in kidney and liver. Low amount of UBC9 is observed in brain, whereas the 18 kDa band of UBC9 is barely visible or absent in heart and skeletal muscle. In heart and muscle extracts the UBC9 antibodies recognizes a 38 kDa protein band,but this band is not visible in extracts of other rat tissues(PMID:14739995).

Protocols

Product Specific Protocols
WB protocol for UBC9 antibody 51018-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

MBio

Mechanism of Adenovirus E4-ORF3-Mediated SUMO Modifications.

Authors - Sook-Young Sohn
humanWB

J Biol Chem

Pathogenic mutations in the Valosin-containing protein/p97(VCP) N-domain inhibit the SUMOylation of VCP and lead to impaired stress response.

Authors - Tao Wang
  • KD Validated
pigWB

Front Immunol

Foot-and-Mouth Disease Virus Counteracts on Internal Ribosome Entry Site Suppression by G3BP1 and Inhibits G3BP1-Mediated Stress Granule Assembly via Post-Translational Mechanisms.

Authors - Xu Ye
human

Autophagy

PDPK1 regulates autophagosome biogenesis by binding to PIK3C3.

Authors - Boli Hu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Marie (Verified Customer) (03-05-2020)

Not working on rat cells/tissus. Working on Humain ( GFP-Ubc9 human overexpression)

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: rat hippocampal culture
UBC9 Antibody Western Blot, validation (1/1000 dilution) in rat hippocampal culture (Cat no:51018-2-AP)