Filter: AllWBIHCIF

WB analysis using 67198-1-Ig (same clone as 67198-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 67198-1-Ig (UBE1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67198-1-PBS in a different storage buffer formulation.
WB analysis using 67198-1-Ig (same clone as 67198-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 67198-1-Ig (UBE1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67198-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 67198-1-Ig (same clone as 67198-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67198-1-Ig (UBE1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67198-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 67198-1-Ig (same clone as 67198-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67198-1-Ig (UBE1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67198-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 67198-1-Ig (same clone as 67198-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67198-1-Ig (UBE1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67198-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 67198-1-Ig (same clone as 67198-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67198-1-Ig (UBE1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67198-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 67198-1-Ig (same clone as 67198-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67198-1-Ig (UBE1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67198-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 67198-1-Ig (same clone as 67198-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67198-1-Ig (UBE1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67198-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 67198-1-Ig (same clone as 67198-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using UBE1 antibody (67198-1-Ig, Clone: 1H7E8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67198-1-PBS in a different storage buffer formulation.

UBE1 Monoclonal antibody, PBS Only

UBE1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 67198-1-PBS

Clone No.1H7E8

Review Product

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

A1S9, A1S9T, A1ST, AMCX1, GXP1, Protein A1S9, SMAX2, UBA1, UBA1A, UBE1, UBE1X, Ubiquitin activating enzyme E1

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

67198-1-PBS targets UBE1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity	Human, mouse, rat
Host / Isotype	Mouse / IgG2a
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag8920 Product name: Recombinant human UBE1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 711-1058 aa of BC013041 Sequence: CHHWHTQYSNNIRQLLHNFPPDQLTSSGAPFWSGPKRCPHPLTFDVNNPLHLDYVMAAANLFAQTYGLTGSQDRAAVATFLQSVQVPEFTPKSGVKIHVSDQELQSANASVDDSRLEELKATLPSPDKLPGFKMYPIDFEKDDDSNFHMDFIVAASNLRAENYDIPSADRHKSKLIAGKIIPAIATTTAAVVGLVCLELYKVVQGHRQLDSYKNGFLNLALPFFGFSEPLAAPRHQYYNQEWTLWDRFEVQGLQPNGEEMTLKQFLDYFKTEHKLEITMLSQGVSMLYSFFMPAAKLKERLDQPMTEIVSRVSKRKLGRHVRALVLELCCNDESGEDVEVPYVRYTIR Predict reactive species
Full Name	ubiquitin-like modifier activating enzyme 1
Calculated Molecular Weight	1058 aa, 118 kDa
Observed Molecular Weight	114-118 kDa
GenBank Accession Number	BC013041
Gene Symbol	UBA1
Gene ID (NCBI)	7317
RRID	AB_2882491
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P22314
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.