Filter: AllWBIHCIF/ICC

WB analysis using 67198-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 67198-1-Ig (UBE1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis using 67198-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 67198-1-Ig (UBE1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
IHC staining of human colon cancer using 67198-1-Ig
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67198-1-Ig (UBE1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 67198-1-Ig
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67198-1-Ig (UBE1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 67198-1-Ig
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67198-1-Ig (UBE1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 67198-1-Ig
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67198-1-Ig (UBE1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 67198-1-Ig
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67198-1-Ig (UBE1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 67198-1-Ig
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67198-1-Ig (UBE1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 67198-1-Ig
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using UBE1 antibody (67198-1-Ig, Clone: 1H7E8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

UBE1 Monoclonal antibody

Name: UBE1 Monoclonal antibody
Brand: Proteintech
SKU: 67198-1-Ig

UBE1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 67198-1-Ig

Clone No.1H7E8

Publications(1)

Review Product

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

A1S9, A1S9T, A1ST, AMCX1, GXP1, Protein A1S9, SMAX2, UBA1, UBA1A, UBE1, UBE1X, Ubiquitin activating enzyme E1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	HeLa cells, HeLa cell, HEK-293 cells, Jurkat cells, HL-60 cells, K-562 cells, HSC-T6 cells, RAW 264.7 cells, NIH/3T3 cells
Positive IHC detected in	human colon cancer tissue, human lung cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:30000
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHC	See 1 publications below

Product Information

67198-1-Ig targets UBE1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity	Human, mouse, rat
Cited Reactivity	human
Host / Isotype	Mouse / IgG2a
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag8920 Product name: Recombinant human UBE1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 711-1058 aa of BC013041 Sequence: CHHWHTQYSNNIRQLLHNFPPDQLTSSGAPFWSGPKRCPHPLTFDVNNPLHLDYVMAAANLFAQTYGLTGSQDRAAVATFLQSVQVPEFTPKSGVKIHVSDQELQSANASVDDSRLEELKATLPSPDKLPGFKMYPIDFEKDDDSNFHMDFIVAASNLRAENYDIPSADRHKSKLIAGKIIPAIATTTAAVVGLVCLELYKVVQGHRQLDSYKNGFLNLALPFFGFSEPLAAPRHQYYNQEWTLWDRFEVQGLQPNGEEMTLKQFLDYFKTEHKLEITMLSQGVSMLYSFFMPAAKLKERLDQPMTEIVSRVSKRKLGRHVRALVLELCCNDESGEDVEVPYVRYTIR Predict reactive species
Full Name	ubiquitin-like modifier activating enzyme 1
Calculated Molecular Weight	1058 aa, 118 kDa
Observed Molecular Weight	114-118 kDa
GenBank Accession Number	BC013041
Gene Symbol	UBA1
Gene ID (NCBI)	7317
RRID	AB_2882491
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P22314
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for UBE1 antibody 67198-1-Ig	Download protocol
IHC protocol for UBE1 antibody 67198-1-Ig	Download protocol
WB protocol for UBE1 antibody 67198-1-Ig	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IHC	Biosci Rep Prognostic value and chemotherapy response prediction of a proliferation essential gene signature in colon cancer Authors - Jinsheng Liu View Article