UBE2B Polyclonal antibody

• 

  WB analysis of L02 using 10733-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 10733-1-AP (UBE2B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Transfected HEK-293 using 10733-1-AP

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 10733-1-AP (UBE2B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary tumor using 10733-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10733-1-AP (UBE2B antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 10733-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10733-1-AP (UBE2B antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

UBE2B Polyclonal Antibody for WB, IHC, ELISA

Cat No. 10733-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Synonyms

E2 17kDa, HHR6B, HR6B, RAD6 homolog B, RAD6B, UBC2, UBE2B, Ubiquitin carrier protein B, Ubiquitin protein ligase B

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Tested Applications

• Positive WB detected in: L02 cells, Transfected HEK-293 cells
• Positive IHC detected in: human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Product Information

10733-1-AP targets UBE2B in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1147

  Product name: Recombinant human UBE2B protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-152 aa of BC005979

  Sequence: MSTPARRRLMRDFKRLQEDPPVGVSGAPSENNIMQWNAVIFGPEGTPFEDGTFKLVIEFSEEYPNKPPTVRFLSKMFHPNVYADGSICLDILQNRWSPTYDVSSILTSIQSLLDEPNPNSPANSQAAQLYQENKREYEKRVSAIVEQSWNDS

• Full Name: ubiquitin-conjugating enzyme E2B (RAD6 homolog)
• Calculated Molecular Weight: 17 kDa
• Observed Molecular Weight: 17 kDa
• GenBank Accession Number: BC005979
• Gene Symbol: UBE2B
• Gene ID (NCBI): 7320
• RRID: AB_2212174
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P63146
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UBE2B (Ubiquitin-conjugating enzyme E2 B) is also named as RAD6B and belongs to the ubiquitin-conjugating enzyme family. Rad6B is an E2-conjugating enzyme, reported to mediate beta-catenin polyubiquitination and stabilization, thus activating the Wnt signaling pathway (PMID:21118991). The gene encodes a 152 amino acid protein. The Rad6B and Cdc34 proteins are significantly elevated in meiotic and postmeiotic haploid germ cells when chromatin modifications occur (PMID:10373550).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB,IF	Biol Reprod; Identification of Ube2b as a Novel Target of Androgen Receptor in Mouse Sertoli Cells.; Authors - Mou Lisha L ; KD Validated
		Int J Mol Med; Identification of NR0B1 as a novel androgen receptor co-repressor in mouse Sertoli cells.; Authors - Yu-Chi Li
human	WB,IHC,IF	Bioengineered; UBE2B promotes ovarian cancer growth via promoting RAD18 mediated ZMYM2 monoubiquitination and stabilization.; Authors - Xi Zeng ; KD Validated
human	WB	Nat Commun; Auranofin targets UBA1 and enhances UBA1 activity by facilitating ubiquitin trans-thioesterification to E2 ubiquitin-conjugating enzymes; Authors - Wenjing Yan