Tested Applications
| Positive WB detected in | HEK-293 cells, mouse brain tissue, mouse kidney tissue, HeLa cells, rat brain tissue, rat kidney tissue |
| Positive IHC detected in | human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15685-1-AP targets UBE2H in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8187 Product name: Recombinant human UBE2H protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-183 aa of BC006277 Sequence: MSSPSPGKRRMDTDVVKLIESKHEVTILGGLNEFVVKFYGPQGTPYEGGVWKVRVDLPDKYPFKSPSIGFMNKIFHPNIDEASGTVCLDVINQTWTALYDLTNIFESFLPQLLAYPNPIDPLNGDAAAMYLHRPEEYKQKIKEYIQKYATEEALKEQEEGTGDSSSESSMSDFSEDEAQDMEL Predict reactive species |
| Full Name | ubiquitin-conjugating enzyme E2H (UBC8 homolog, yeast) |
| Calculated Molecular Weight | 183 aa, 21 kDa |
| Observed Molecular Weight | 21 kDa |
| GenBank Accession Number | BC006277 |
| Gene Symbol | UBE2H |
| Gene ID (NCBI) | 7328 |
| RRID | AB_10858632 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P62256 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for UBE2H antibody 15685-1-AP | Download protocol |
| WB protocol for UBE2H antibody 15685-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The UBE2H Polyclonal antibody (Cat# 15685-1-AP) has 5 citations published in Nature Communications, Molecular Cell, Cell Reports, Frontiers in Cardiovascular Medicine, and bioRxiv. The research spans mTORC1–CTLH E3 ligase signaling, ubiquitin-mediated proteolysis, intracellular Mycobacterium tuberculosis growth in macrophages, pyrimidine salvage pathway regulation, and cardiac valve interstitial cell calcification. All citations used the antibody for Western blot in human, mouse, or rat samples.
| Species | Application | Title |
|---|---|---|
Nat Commun Genome-wide screen of Mycobacterium tuberculosis-infected macrophages revealed GID/CTLH complex-mediated modulation of bacterial growth | ||
Mol Cell mTORC1-CTLH E3 ligase regulates the degradation of HMG-CoA synthase 1 through the Pro/N-degron pathway | ||
Cell Rep mTORC1 regulates the pyrimidine salvage pathway by controlling UCK2 turnover via the CTLH-WDR26 E3 ligase | ||
Front Cardiovasc Med Nutrient restriction protects against valve interstitial cell calcification by upregulating ubiquitin mediated proteolysis | ||
bioRxiv Genome-wide screen of Mycobacterium tuberculosis- infected macrophages identified the GID/CTLH complex as a determinant of intracellular bacterial growth |
Reviews
What customers say
The sole review was negative. In a Western blot for UBE2H in HEK293T cells, the antibody produced a very weak target band and multiple nonspecific bands. The reviewer used a Bis-Tris gel, PVDF membrane, and overnight primary antibody incubation at 4°C with a 1:1000 dilution. Overall, the reported performance was unsatisfactory under these conditions.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
H (Verified Customer) (09-27-2020) | My attempt to use this antibody to detect UBE2H in HEK293T failed. The band intensity was very low, and there were many non-specific bands as well. I used bis-Tris gel and transferred the proteins to PVDF membrane. Primary antibody was incubated over night at 4'C.
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