UBE2Q2 Polyclonal antibody

UBE2Q2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 12581-1-AP

Synonyms

UBCi, UBE2Q2, Ubiquitin carrier protein Q2, Ubiquitin protein ligase Q2

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Validation Data Gallery

• 

  WB analysis of A431 using 12581-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 12581-1-AP (UBE2Q2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293T using 12581-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 12581-1-AP (UBE2Q2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse ovary using 12581-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 12581-1-AP (UBE2Q2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 12581-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 12581-1-AP (UBE2Q2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse ovary using 12581-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 12581-1-AP (UBE2Q2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293T using 12581-1-AP

  IP result of anti-UBE2Q2 (IP:12581-1-AP, 4ug; Detection:12581-1-AP 1:300) with HEK-293T cells lysate 1320 ug.

• 

  IHC staining of human skin cancer using 12581-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer using 12581-1-AP (UBE2Q2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human skin cancer using 12581-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer using 12581-1-AP (UBE2Q2 antibody) at dilution of 1:100 (under 10x lens).

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Tested Applications

• Positive WB detected in: A431 cells, mouse ovary tissue, HEK-293T cells
• Positive IP detected in: HEK-293T cells
• Positive IHC detected in: human skin cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

12581-1-AP targets UBE2Q2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: UBE2Q2 fusion protein Ag3276
• Full Name: ubiquitin-conjugating enzyme E2Q family member 2
• Calculated Molecular Weight: 375 aa, 43 kDa
• Observed Molecular Weight: 43 kDa
• GenBank Accession Number: BC017708
• Gene Symbol: UBE2Q2
• Gene ID (NCBI): 92912
• RRID: AB_2256807
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Cell; In vivo structural characterization of the SARS-CoV-2 RNA genome identifies host proteins vulnerable to repurposed drugs.; Authors - Lei Sun
mouse	WB	Transl Cancer Res; Proteome-wide identification and functional analysis of ubiquitinated proteins in Hepa1-6 cells by knockdown of E3 ubiquitin ligase SIAH1; Authors - Xiao Hu