UBE2S Recombinant monoclonal antibody, PBS Only

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  WB analysis using 81854-1-RR (same clone as 81854-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 81854-1-RR (UBE2S antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81854-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 81854-1-RR (same clone as 81854-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 81854-1-RR (UBE2S antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 81854-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 81854-1-RR (same clone as 81854-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 81854-1-RR (UBE2S antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 81854-1-PBS in a different storage buffer formulation.

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  IF Staining of NCCIT using 81854-1-RR (same clone as 81854-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed NCCIT cells using UBE2S antibody (81854-1-RR, Clone: 6D18 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81854-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 81854-1-RR

  Biolayer interferometry (BLl) kinetic assays of 81854-1-RR against Human UBE2S were performed. The affinity constant is below 1 pM.

UBE2S Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 81854-1-PBS

Clone No.6D18

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

6D18, E2 EPF, E2EPF, E2-EPF, EC:2.3.2.23

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Product Information

81854-1-PBS targets UBE2S in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag5264

  Product name: Recombinant human UBE2S protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-222 aa of BC066948

  Sequence: MNSNVENLPPHIIRLVYKEVTTLTADPPDGIKVFPNEEDLTDLQVTIEGPEGTPYAGGLFRMKLLLGKDFPASPPKGYFLTKIFHLNVGANGEICVNVLKRDWTAELGIRHVLLTIRCLLIHPNPESALNEEAGRLLLENSEEYAARARLLTEIHGGAGGPSGRAEAGRALASGTAASSTDPGAPGGLGGAEGPMAKKHAGERDKKLAAKKKTDKKRALRRL

• Full Name: ubiquitin-conjugating enzyme E2S
• Calculated Molecular Weight: 222 aa, 24 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: BC066948
• Gene Symbol: UBE2S
• Gene ID (NCBI): 27338
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q16763
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

UBE2S(Ubiquitin-conjugating enzyme E2 S) is also named as E2EPF and belongs to the ubiquitin-conjugating enzyme family. It acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C) and a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Overexpression of UBE2S can increase tumor cell proliferation, invasion, and metastasis through the VHL-HIF pathway (PMID:16819549).