UBE2V1 Polyclonal antibody

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  WB analysis of human spleen using 10207-2-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 10207-2-AP (UBE2V1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of BxPC-3 using 10207-2-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 10207-2-AP (UBE2V1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

UBE2V1 Polyclonal Antibody for WB, ELISA

Cat No. 10207-2-AP

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

CIR1, CROC 1, CROC1, UBE2V, UBE2V1, UEV 1, UEV1, UEV1A

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Tested Applications

• Positive WB detected in: human spleen tissue, BxPC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

10207-2-AP targets UBE2V1 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag0278

  Product name: Recombinant human UBE2V1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 17-109 aa of BC000468

  Sequence: LLEELEEGQKGVGDGTVSWGLEDDEDMTLTRWTGMIIGPPRTIYENRIYSLKIECGPKYPEAPPFVRFVTKINMNGVNSSNGVVDPRAISVLA

• Full Name: ubiquitin-conjugating enzyme E2 variant 1
• Calculated Molecular Weight: 20 kDa
• Observed Molecular Weight: 20 kDa
• GenBank Accession Number: BC000468
• Gene Symbol: UBE2V1
• Gene ID (NCBI): 7335
• RRID: AB_2272560
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q13404
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UBE2V1(Ubiquitin-conjugating enzyme E2 variant 1) is also named as CROC1, UBE2V, UEV1 and belongs to the ubiquitin-conjugating enzyme family. It has no ubiquitin ligase activity on its own and the UBE2V1-UBE2N heterodimer catalyzes the synthesis of non-canonical poly-ubiquitin chains that are linked through Lys-63. CROC1 transcripts in all human tissues examined, with highest levels in brain, skeletal muscle, and kidney(PMID:9305758). It has 5 isoforms produced by alternative splicing.

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Sarah (Verified Customer) (09-05-2025)

Antibody signal was quite weak in HEK293T cells, including some off-target products as well. Antibody was used in 5% BSA in milk with overnight incubation at 4C.

• Applications: Western Blot
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: HEK293T