Various lysates were subjected to SDS PAGE followed by western blot with 84154-4-RR (UCHL1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 84154-4-RR (UCHL1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
IHC staining of mouse brain using 84154-4-RR
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84154-4-RR (UCH-L1/PGP9.5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with .
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84154-4-RR (UCH-L1/PGP9.5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with .
IF Staining of mouse brain using 84154-4-RR
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using UCH-L1/PGP9.5 antibody (84154-4-RR, Clone: 241107B2 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using UCH-L1/PGP9.5 antibody (84154-4-RR, Clone: 241107B2 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
IF Staining of Neuro-2a using 84154-4-RR
Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using UCH-L1/PGP9.5 antibody (84154-4-RR, Clone: 241107B2 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using UCH-L1/PGP9.5 antibody (84154-4-RR, Clone: 241107B2 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
Affinity and Kinetic Characterization of 84154-4-RR
Biolayer interferometry (BLl) kinetic assays of 84154-4-RR against Human UCHL1/PGP9.5 were performed. The affinity constant is below 1 pM.
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Neuro-2a cells, SH-SY5Y cells, mouse brain tissue, rat brain tissue
Positive IHC detected in
mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected in
mouse brain tissue
Positive IF/ICC detected in
Neuro-2a cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:5000-1:50000
Immunohistochemistry (IHC)
IHC : 1:50-1:500
Immunofluorescence (IF)-P
IF-P : 1:125-1:500
Immunofluorescence (IF)/ICC
IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
84154-4-RR targets UCH-L1/PGP9.5 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
UCHL1(Ubiquitin carboxyl-terminal hydrolase isozyme L1) is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. Expression of UCHL1 is highly specific to neurons and to cells of the diffuse neuroendocrine system and their tumors. It is present in all neurons(PMID: 6343558). This protein is present in brain at concentrations at least 50 times greater than in other organs and is a major protein component of neuronal cytoplasm (PMID:7217993). And UCHL1 is a Parkinson's disease susceptibility gene (PMID:15048890).
Various lysates were subjected to SDS PAGE followed by western blot with 84154-4-RR (UCHL1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of mouse brain using 84154-4-RR
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84154-4-RR (UCH-L1/PGP9.5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with .
IF-P Figures
IF Staining of mouse brain using 84154-4-RR
Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using UCH-L1/PGP9.5 antibody (84154-4-RR, Clone: 241107B2 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
IF/ICC Figures
IF Staining of Neuro-2a using 84154-4-RR
Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using UCH-L1/PGP9.5 antibody (84154-4-RR, Clone: 241107B2 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
AFFINITY Figures
Affinity and Kinetic Characterization of 84154-4-RR
Biolayer interferometry (BLl) kinetic assays of 84154-4-RR against Human UCHL1/PGP9.5 were performed. The affinity constant is below 1 pM.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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