Tested Applications
| Positive WB detected in | HeLa cells, rat spleen tissue, Jurkat cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12384-1-AP targets UCHL3 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3053 Product name: Recombinant human UCHL3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-230 aa of BC018125 Sequence: MEGQRWLPLEANPEVTNQFLKQLGLHPNWQFVDVYGMDPELLSMVPRPVCAVLLLFPITEKYEVFRTEEEEKIKSQGQDVTSSVYFMKQTISNACGTIGLIHAIANNKDKMHFESGSTLKKFLEESVSMSPEERARYLENYDAIRVTHETSAHEGQTEAPSIDEKVDLHFIALVHVDGHLYELDGRKPFPINHGETSDETLLEDAIEVCKKFMERDPDELRFNAIALSAA Predict reactive species |
| Full Name | ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase) |
| Calculated Molecular Weight | 26 kDa |
| Observed Molecular Weight | 26 kDa |
| GenBank Accession Number | BC018125 |
| Gene Symbol | UCHL3 |
| Gene ID (NCBI) | 7347 |
| RRID | AB_2210915 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P15374 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
UCH-L3 belongs to the ubiquitin C-terminal hydrolase family that deubiquitinates ubiquitin-protein conjugates in the ubiquitin-proteasome system. This enzyme indirectly increases the phosphorylation of IGFIR, AKT, FOXO1 and promotes insulin-signaling and insulin-induced adipogenesis. It is required for stress-response retinal, skeletal muscle and germ cell maintenance and may be involved in working memory.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for UCHL3 antibody 12384-1-AP | Download protocol |
| IP protocol for UCHL3 antibody 12384-1-AP | Download protocol |
| WB protocol for UCHL3 antibody 12384-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The UCHL3 Polyclonal antibody (Cat# 12384-1-AP) has 26 citations. These appear in journals including Nature Communications, Autophagy, Molecular Cell, Advanced Science, Cell Death & Differentiation, Oncogene, Free Radical Biology and Medicine, Genes & Development, Science Reports, and Cancer Letters. Research fields span ubiquitin signaling and deubiquitination, DNA repair, autophagy, ferroptosis, and cuproptosis, plus cancer biology (hepatocellular, gastric, colorectal, ovarian, thyroid, pancreatic, and breast cancers), innate immunity, and protein homeostasis.
| Species | Application | Title |
|---|---|---|
Autophagy MLKL-USP7-UBA52 signaling is indispensable for autophagy in brain through maintaining ubiquitin homeostasis | ||
Nat Commun Structural basis for specific inhibition of the deubiquitinase UCHL1
| ||
Nat Commun Farrerol directly activates the deubiqutinase UCHL3 to promote DNA repair and reprogramming when mediated by somatic cell nuclear transfer
| ||
Adv Sci (Weinh) UCHL3 Regulates Subgenomic Flaviviral RNA Condensates to Promote Virus Propagation.
| ||
Adv Sci (Weinh) p300 Degradation by the p53-SIAH1 Axis Relieves TBK1 Acetylation to Enhance Innate Antiviral Immunity. |
