UCP2 Polyclonal antibody

• Featured Product
• KD/KO Validated

UCP2 Polyclonal Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

65

Reviews (2)

Cat no : 11081-1-AP

Synonyms

UCPH, UCP 2, SLC25A8, Dicarboxylate carrier SLC25A8, BMIQ4

---

Validation Data Gallery

• 

  WB analysis using 11081-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11081-1-AP (UCP2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 11081-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 11081-1-AP (UCP2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 11081-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11081-1-AP (UCP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 11081-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 11081-1-AP (UCP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse skeletal muscle using 11081-1-AP

  IP result of anti-UCP2 (IP:11081-1-AP, 4ug; Detection:11081-1-AP 1:500) with mouse skeletal muscle tissue lysate 4000ug.

• 

  IHC staining of human cervical cancer using 11081-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 11081-1-AP (UCP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human hepatocirrhosis using 11081-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 11081-1-AP (UCP2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human hepatocirrhosis using 11081-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 11081-1-AP (UCP2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 11081-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 11081-1-AP (UCP2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 11081-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 11081-1-AP (UCP2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon cancer using 11081-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11081-1-AP (UCP2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 11081-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11081-1-AP (UCP2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skeletal muscle using 11081-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 11081-1-AP (UCP2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 11081-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 11081-1-AP (UCP2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary using 11081-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 11081-1-AP (UCP2 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human ovary using 11081-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tissue slide using 11081-1-AP (UCP2 Antibody) at dilution of 1:200 (under 40x lens).

• 

  FC experiment of HeLa using 11081-1-AP

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug UCP2 Polyclonal antibody (11081-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Tested Applications

• Positive WB detected in: mouse adipose tissue, mouse skeletal muscle tissue, mouse heart tissue, mouse spleen tissue, mouse liver tissue, rat liver tissue
• Positive IHC detected in: human cervical cancer tissue, human ovary tissue, human hepatocirrhosis tissue, human skeletal muscle tissue, human liver tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: HeLa cells

It is strongly recommended that fresh-made lysate be used for western blot assays.

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 56 publications below
• IHC: See 5 publications below
• IF: See 11 publications below

Product Information

11081-1-AP targets UCP2 in WB, IHC, IF, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: UCP2 fusion protein Ag1551
• Full Name: uncoupling protein 2 (mitochondrial, proton carrier)
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 28-30 kDa
• GenBank Accession Number: BC011737
• Gene Symbol: UCP2
• Gene ID (NCBI): 7351
• RRID: AB_2213793
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UCP2 (uncoupling protein 2) is a member of the mitochondrial carrier protein super family located in the mitochondrial inner membrane. The UCPs differ greatly in tissue distribution: UCP1 is expressed only in brown adipose; UCP3 preferentially in skeletal muscle, brown adipose and heart; UCP4 and UCP5 mainly in the nervous system (18167556). In contract, UCP2 has a broad distribution and is implicated in a wide range of pathophysiological processes, including diabetes and cancer. This antibody detect a band around 28-33 kDa corresponding to UCP2 protein in mouse liver, skeletal muscle and pancreas. Some upper and lower bands also were observed due to the dimer formation and degradation of the UCP2 protein (20103532). This antibody was raised against the full-length UCP2 protein and may cross-react with UCP3. It is notable that UCP2 is known to have a very short half-life less than 1 hour (17240372). It is strongly recommended that fresh-made lysate be used for western blot assays.

Publications

Species	Application	Title
mouse	WB	Sci Transl Med; Irisin protects mitochondria function during pulmonary ischemia/reperfusion injury.; Authors - Ken Chen
mouse	WB	J Thromb Haemost; The plasminogen receptor Plg-RKT regulates adipose function and metabolic homeostasis.; Authors - Fahumiya Samad
human	WB	EMBO Mol Med; Disease-specific phenotypes in iPSC-derived neural stem cells with POLG mutations.; Authors - Kristina Xiao Liang
rat	WB	Metabolism; Far-infrared radiation prevents decline in β-cell mass and function in diabetic mice via the mitochondria-mediated Sirtuin1 pathway.; Authors - Yung-Ho Hsu
mouse	IHC	Stem Cells; UCP2 Regulates Embryonic Neurogenesis via ROS-Mediated Yap Alternation in the Developing Neocortex.; Authors - Fen Ji
mouse	WB,IF	Sci Rep; Genipin inhibits NLRP3 and NLRC4 inflammasome activation via autophagy suppression.; Authors - Shui-Xing Yu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

David (Verified Customer) (06-03-2022)

Works excellent, I recommend.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: brain

UCP2 Antibody Immunofluorescence validation (1:50 dilution) in brain (Cat no:11081-1-AP)

FH

Mohammad (Verified Customer) (06-28-2019)

it is working excellent, I recommend it.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mouse hippocampus

Related Products

PPARD Polyclonal antibody

10156-2-AP

PPAR Gamma Polyclonal antibody

16643-1-AP

UCP1 Polyclonal antibody

23673-1-AP

UCP3 Polyclonal antibody

10750-1-AP

UCP5 Polyclonal antibody

25223-1-AP