UFD1L Polyclonal antibody

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• UFD1L Polyclonal antibody (10615-1-AP)

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• KD/KO Validated

UFD1L Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 10615-1-AP Copied!

Publications (24)

Reviews (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Synonyms

UB fusion protein 1, Ubiquitin fusion degradation protein 1, Ubiquitin recognition factor in ER-associated degradation protein 1, UFD1

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  WB analysis of K-562 using 10615-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10615-1-AP (UFD1L antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  WB analysis of HeLa using 10615-1-AP

  WB result of UFD1L antibody (10615-1-AP; 1:15000; incubated at room temperature for 1.5 hours) with sh-Control and sh-UFD1L transfected HeLa cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  WB analysis of mouse thymus using 10615-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 10615-1-AP (UFD1L Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  WB analysis of human testis using 10615-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 10615-1-AP (UFD1L antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HepG2 using 10615-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10615-1-AP (UFD1L Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of Raji using 10615-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 10615-1-AP (UFD1L Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of Raji using 10615-1-AP

  IP result of anti-UFD1L (IP:10615-1-AP, 4ug; Detection:10615-1-AP 1:1000) with Raji cells lysate 2000ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  IHC staining of human stomach using 10615-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10615-1-AP (UFD1L antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse kidney using 10615-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10615-1-AP (UFD1L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 10615-1-AP

  Immunofluorescent analysis of HeLa cells using 10615-1-AP (UFD1L antibody) at dilution of 1:25 and Alexa Fluor 594-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HepG2 using 10615-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using UFD1L antibody (10615-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of HEK-293 using 10615-1-AP

  1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human UFD1L (10615-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: K-562 cells, HeLa cells, HepG2 cells, human testis tissue, mouse thymus tissue, Raji cells
• Positive IP detected in: Raji cells
• Positive IHC detected in: human stomach tissue, mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells, HeLa cells
• Positive FC (Intra) detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

10615-1-AP targets UFD1L in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag0954

  Product name: Recombinant human UFD1L protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-307 aa of BC005087

  Sequence: MFSFNMFDHPIPRVFQNRFSTQYRCFSVSMLAGPNDRSDVEKGGKIIMPPSALDQLSRLNITYPMLFKLTNKNSDRMTHCGVLEFVADEGICYLPHWMMQNLLLEEGGLVQVESVNLQVATYSKFQPQSPDFLDITNPKAVLENALRNFACLTTGDVIAINYNEKIYELRVMETKPDKAVSIIECDMNVDFDAPLGYKEPERQVQHEESTEGEADHSGYAGELGFRAFSGSGNRLDGKKKGVEPSPSPIKPGDIKRGIPNYEFKLGKITFIRNSRPLVKKVEEDEAGGRFVAFSGEGQSLRKKGRKP

• Full Name: ubiquitin fusion degradation 1 like (yeast)
• Calculated Molecular Weight: 35 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC005087
• Gene Symbol: UFD1L
• Gene ID (NCBI): 7353
• RRID: AB_2213944
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q92890
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The UFD1L Polyclonal antibody (Cat# 10615-1-AP) has 24 citations spanning journals including Nature, Science, Nature Communications, Developmental Cell, Cell Reports, Science Signaling, Cell Signaling, Journal of Biological Chemistry, and eLife. Research fields covered include nuclear envelope reformation, ESCRT machinery, VCP/p97 ubiquitination, ERAD, autophagy, stress granules, proteasome-mediated degradation, and cancer biology (breast, prostate, oral). Applications cited include WB, IP, IF, and IHC.

Automatically generated from the 24 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Nature; ESCRT-III controls nuclear envelope reformation.; Authors - Yolanda Olmos ; KD Validated
human	IP	Science; Ubiquitination of G3BP1 mediates stress granule disassembly in a context-specific manner.; Authors - Youngdae Gwon
		Nat Commun; A UFD1 variant encoding a microprotein modulates UFD1f and IPMK ubiquitination to play pivotal roles in anti-stress responses; Authors - Xiuzhi Li
human	WB	Nat Commun; Cell-type-specific functionality encoded within the intrinsically disordered regions of OCT4.; Authors - Burak Ozkan
human	WB	Dev Cell; The ESCRT machinery counteracts Nesprin-2G-mediated mechanical forces during nuclear envelope repair.; Authors - Samuel S Wallis ; KD Validated
human	IF	Cell Rep; Yeast Smy2 and its human homologs GIGYF1 and -2 regulate Cdc48/VCP function during transcription stress; Authors - Michelle Harreman Lehner

Reviews

5.0 out of 5

1 verified customer reviews

What customers say

The single available review rates this antibody 5 stars. The user reports it performed excellently for Western blotting, using a 1:1000 dilution on skeletal muscle tissue. Overall, the feedback is positive, highlighting strong and reliable WB performance.

Automatically generated from the 1 verified customer reviews below by Able AI.

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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Leslie (Verified Customer) (10-08-2019)

Excellent antibody for Western blots

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Skeletal Muscle

UFD1L Antibody Western Blot, validation (1:1000 dilution) in Skeletal Muscle (Cat no:10615-1-AP)