UNC13D Monoclonal antibody

UNC13D Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1C5C1

Cat no : 67193-1-Ig

Synonyms

FHL3, HLH3, HPLH3, Munc13 4, Protein unc 13 homolog D, unc 13 homolog D (C. elegans), UNC13D

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Validation Data Gallery

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  WB analysis using 67193-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67193-1-Ig (UNC13D antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW 264.7 using 67193-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 67193-1-Ig (UNC13D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig spleen using 67193-1-Ig

  pig spleen tissue were subjected to SDS PAGE followed by western blot with 67193-1-Ig (UNC13D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat spleen using 67193-1-Ig

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 67193-1-Ig (UNC13D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of rat spleen using 67193-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 67193-1-Ig (UNC13D antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat spleen using 67193-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 67193-1-Ig (UNC13D antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: THP-1 cells, RAW 264.7 cells, pig spleen tissue, rat spleen tissue, K-562 cells, Jurkat cells, HL-60 cells
• Positive IHC detected in: rat spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:10000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67193-1-Ig targets UNC13D in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat, pig samples.

• Tested Reactivity: Human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: UNC13D fusion protein Ag10497
• Full Name: unc-13 homolog D (C. elegans)
• Calculated Molecular Weight: 1090 aa, 103 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: BC067084
• Gene Symbol: UNC13D
• Gene ID (NCBI): 201294
• RRID: AB_2882488
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.