CoraLite® Plus 488-conjugated UNC13D Monoclonal antibody

UNC13D Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1C5C1

Cat no : CL488-67193

Synonyms

FHL3, HLH3, HPLH3, Munc13 4, Protein unc 13 homolog D, unc 13 homolog D (C. elegans), UNC13D

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Validation Data Gallery

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  WB analysis using CL488-67193

  Various lysates were subjected to SDS PAGE followed by western blot with CL488-67193 (UNC13D antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: Jurkat cells, HL-60 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67193 targets UNC13D in WB applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: UNC13D fusion protein Ag10497
• Full Name: unc-13 homolog D (C. elegans)
• Calculated Molecular Weight: 1090 aa, 103 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: BC067084
• Gene Symbol: UNC13D
• Gene ID (NCBI): 201294
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UNC13D is a member of the UNC13 family, containing similar domain structure as other family members but lacking an N-terminal phorbol ester-binding C1 domain present in other Munc13 proteins. The protein appears to play a role in vesicle maturation during exocytosis and is involved in regulation of cytolytic granules secretion.