UQCRC1 Recombinant monoclonal antibody

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• UQCRC1 Recombinant monoclonal antibody (83671-2-RR)

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• KD/KO Validated

UQCRC1 Uni-rAb^® Recombinant Antibody for WB, IHC, IF-P, ELISA

Cat No. 83671-2-RR Copied!

Clone No. 240507A6

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

240507A6, Complex III subunit 1, Core protein I, Cytochrome b-c1 complex subunit 1, mitochondrial, D3S3191

• 

  WB analysis using 83671-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83671-2-RR (UQCRC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 83671-2-RR

  WB result of UQCRC1 antibody (83671-2-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-UQCRC1 transfected HeLa cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human stomach cancer using 83671-2-RR

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 83671-2-RR (UQCRC1 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of mouse brain using 83671-2-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using UQCRC1 antibody (83671-2-RR, Clone: 240507A6 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Affinity and Kinetic Characterization of 83671-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83671-2-RR against Human UQCRC1 were performed. The affinity constant is 0.294 nM.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells, L02 cells, HepG2 cells, mouse brain tissue, mouse liver tissue, rat brain tissue, rat liver tissue
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83671-2-RR targets UQCRC1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag16322

  Product name: Recombinant human UQCRC1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 159-480 aa of BC009586

  Sequence: SQIEKERDVILREMQENDASMRDVVFNYLHATAFQGTPLAQAVEGPSENVRKLSRADLTEYLSTHYKAPRMVLAAAGGVEHQQLLDLAQKHLGGIPWTYAEDAVPTLTPCRFTGSEIRHRDDALPFAHVAIAVEGPGWASPDNVALQVANAIIGHYDCTYGGGVHLSSPLASGAVANKLCQSFQTFSICYAETGLLGAHFVCDRMKIDDMMFVLQGQWMRLCTSATESEVARGKNILRNALVSHLDGTTPVCEDIGRSLLTYGRRIPLAEWESRIAEVDASVVREICSKYIYDQCPAVAGYGPIEQLPDYNRIRSGMFWLRF

• Full Name: ubiquinol-cytochrome c reductase core protein I
• Calculated Molecular Weight: 480 aa, 53 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC009586
• Gene Symbol: UQCRC1
• Gene ID (NCBI): 7384
• RRID: AB_3671276
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P31930
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UQCRC1(Cytochrome b-c1 complex subunit 1, mitochondrial), also named as QCR1 and UQCR1, is a ubunit of the cytochrome bc1 complex (complex III) of the mitochondrial respiratory chain, may mediate the formation of the complex between cytochromes c and c1. The gene encodes a 53 kDa protein with a 34 amino acids transit peptide. It is associated with apoptosis or inhibition of cancer cell growth.

Publications

What published studies show

The UQCRC1 Recombinant monoclonal antibody (240507A6), catalog number 83671-2-RR, has 1 total citation. It appears in Food and Chemical Toxicology, a study investigating brominated flame retardants and their potential targeting of UQCRC1 and NR3C2 in inflammatory bowel disease (IBD) and colorectal cancer (CRC). The associated research fields include toxicology, gastrointestinal disease, and oncology.

Automatically generated from the 1 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
		Food Chem Toxicol; Brominated flame retardants: UQCRC1 and NR3C2 as potential targets in IBD and CRC.; Authors - Jianhai Wu