Tested Applications
| Positive WB detected in | HeLa cells, pig brain tissue, human placenta tissue, pig heart tissue, pig kidney, rat brain tissue, mouse brain tissue |
| Positive IHC detected in | human colon cancer tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67547-1-Ig targets UQCRC2 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6874 Product name: Recombinant human UQCRC2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 103-453 aa of BC000484 Sequence: IEAVGGKLSVTATRENMAYTVECLRGDVDILMEFLLNVTTAPEFRRWEVADLQPQLKIDKAVAFQNPQTHVIENLHAAAYRNALANPLYCPDYRIGKVTSEELHYFVQNHFTSARMALIGLGVSHPVLKQVAEQFLNMRGGLGLSGAKANYRGGEIREQNGDSLVHAAFVAESAVAGSAEANAFSVLQHVLGAGPHVKRGSNTTSHLHQAVAKATQQPFDVSAFNASYSDSGLFGIYTISQATAAGDVIKAAYNQVKTIAQGNLSNTDVQAAKNKLKAGYLMSVESSECFLEEVGSQALVAGSYMPPSTVLQQIDSVANADIINAAKKFVSGQKSMAASGNLGHTPFVDEL Predict reactive species |
| Full Name | ubiquinol-cytochrome c reductase core protein II |
| Calculated Molecular Weight | 48 kDa |
| Observed Molecular Weight | 48 kDa |
| GenBank Accession Number | BC000484 |
| Gene Symbol | UQCRC2 |
| Gene ID (NCBI) | 7385 |
| RRID | AB_2882763 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P22695 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for UQCRC2 antibody 67547-1-Ig | Download protocol |
| IHC protocol for UQCRC2 antibody 67547-1-Ig | Download protocol |
| WB protocol for UQCRC2 antibody 67547-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The UQCRC2 Monoclonal antibody (1A5G11), catalog number 67547-1-Ig, has 11 citations. These appear in journals including Circulation Research, Experimental & Molecular Medicine, Cell Death & Disease, Phytomedicine, Science of the Total Environment, Journal of Translational Medicine, Phytotherapy Research, Food & Function, European Journal of Pharmacology, and International Journal of Molecular Sciences. The research fields span pulmonary hypertension, osteoclastogenesis, hepatocellular carcinoma, mitochondrial metabolism, renal fibrosis, neurodevelopment, glioma, thermogenesis, ferroptosis, NAFLD, and bladder dysfunction.
| Species | Application | Title |
|---|---|---|
Exp Mol Med Osteoblast-derived osteomodulin restrains osteoclastogenesis via ITGB8/RRM2-mediated reduction of mitochondrial respiration and mitochondrial ATP production. | ||
Cell Death Dis BNIP3-mediated mitophagy boosts the competitive growth of Lenvatinib-resistant cells via energy metabolism reprogramming in HCC | ||
Phytomedicine Kang Shuai Lao Pian exerts anti-aging effects by enhancing mitochondrial oxidative metabolism. | ||
Phytomedicine Zhen Wu decoction represses renal fibrosis by invigorating tubular NRF2 and TFAM to fuel mitochondrial bioenergetics | ||
Sci Total Environ Fluoride impairs mitochondrial translation by targeting miR-221-3p/c-Fos/RMND1 axis contributing to neurodevelopment defects |
