UQCRFS1 Recombinant monoclonal antibody, PBS Only

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• UQCRFS1 Recombinant monoclonal antibody, PBS Only (88500-1-PBS)

UQCRFS1 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, IP, Indirect ELISA

Cat No. 88500-1-PBS

Clone No.261156A1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, Indirect ELISA

Synonyms

Complex III subunit 5, Complex III subunit IX, Cytochrome b-c1 complex subunit 5, Cytochrome b-c1 complex subunit 9, Cytochrome b-c1 complex subunit Rieske, mitochondrial

• 

  WB analysis using 88500-1-RR (same clone as 88500-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 88500-1-RR (UQCRFS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 88500-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HeLa using 88500-1-RR (same clone as 88500-1-PBS)

  IP result of anti-UQCRFS1 (IP:88500-1-RR, 4ug; Detection:88500-1-RR 1:3000) with HeLa cells lysate 1080 ug. This data was developed using the same antibody clone with 88500-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of MCF-7 using 88500-1-RR (same clone as 88500-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using UQCRFS1 antibody (88500-1-RR, Clone: 261156A1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 88500-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Product Information

88500-1-PBS targets UQCRFS1 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag13315

  Product name: Recombinant human UQCRFS1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-274 aa of BC010035

  Sequence: MLSVAARSGPFAPVLSATSRGVAGALRPLVQATVPATPEQPVLDLKRPFLSRESLSGQAVRRPLVASVGLNVPASVCYSHTDIKVPDFSEYRRLEVLDSTKSSRESSEARKGFSYLVTGVTTVGVAYAAKNAVTQFVSSMSASADVLALAKIEIKLSDIPEGKNMAFKWRGKPLFVRHRTQKEIEQEAAVELSQLRDPQHDLDRVKKPEWVILIGVCTHLGCVPIANAGDFGGYYCPCHGSHYDASGRIRLGPAPLNLEVPTYEFTSDDMVIVG

• Full Name: ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1
• Calculated Molecular Weight: 30 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC010035
• Gene Symbol: UQCRFS1
• Gene ID (NCBI): 7386
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P47985
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

The ubiquinol cytochrome c reductase UQCRFS1 is a key subunit of the cytochrome bc1 complex (complex III) of the mitochondrial respiratory chain. The UQCRFS1 gene encode a 30 kDa protein with a transit peptide and it appears to be involved in development of more aggressive phenotype of breast cancer(PMID:15047214). UQCRFS1 is also named as RISP, complex III subunit 5, cytochrome b-c1 complex subunit 5 and rieske iron-sulfur protein.