UQCRFS1 Recombinant monoclonal antibody

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• UQCRFS1 Recombinant monoclonal antibody (88500-1-RR)

UQCRFS1 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, IP, ELISA

Cat No. 88500-1-RR

Clone No.261156A1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Synonyms

Complex III subunit 5, Complex III subunit IX, Cytochrome b-c1 complex subunit 5, Cytochrome b-c1 complex subunit 9, Cytochrome b-c1 complex subunit Rieske, mitochondrial

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  WB analysis using 88500-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 88500-1-RR (UQCRFS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  IP experiment of HeLa using 88500-1-RR

  IP result of anti-UQCRFS1 (IP:88500-1-RR, 4ug; Detection:88500-1-RR 1:3000) with HeLa cells lysate 1080 ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of MCF-7 using 88500-1-RR

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using UQCRFS1 antibody (88500-1-RR, Clone: 261156A1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells, MCF-7 cells, A2780 cells, mouse heart tissue, rat heart tissue, human heart tissue, human placenta
• Positive IP detected in: HeLa cells
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

88500-1-RR targets UQCRFS1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag13315

  Product name: Recombinant human UQCRFS1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-274 aa of BC010035

  Sequence: MLSVAARSGPFAPVLSATSRGVAGALRPLVQATVPATPEQPVLDLKRPFLSRESLSGQAVRRPLVASVGLNVPASVCYSHTDIKVPDFSEYRRLEVLDSTKSSRESSEARKGFSYLVTGVTTVGVAYAAKNAVTQFVSSMSASADVLALAKIEIKLSDIPEGKNMAFKWRGKPLFVRHRTQKEIEQEAAVELSQLRDPQHDLDRVKKPEWVILIGVCTHLGCVPIANAGDFGGYYCPCHGSHYDASGRIRLGPAPLNLEVPTYEFTSDDMVIVG

• Full Name: ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1
• Calculated Molecular Weight: 30 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC010035
• Gene Symbol: UQCRFS1
• Gene ID (NCBI): 7386
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P47985
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The ubiquinol cytochrome c reductase UQCRFS1 is a key subunit of the cytochrome bc1 complex (complex III) of the mitochondrial respiratory chain. The UQCRFS1 gene encode a 30 kDa protein with a transit peptide and it appears to be involved in development of more aggressive phenotype of breast cancer(PMID:15047214). UQCRFS1 is also named as RISP, complex III subunit 5, cytochrome b-c1 complex subunit 5 and rieske iron-sulfur protein.