URAT1 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 82964-1-RR (same clone as 82964-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82964-1-RR (URAT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82964-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HepG2 using 82964-1-RR (same clone as 82964-1-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.4 ug URAT1 Recombinant antibody (82964-1-RR, Clone:230204B3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 82964-1-PBS in a different storage buffer formulation.

URAT1 Uni-rAb^® Recombinant Antibody for WB, FC (Intra), Indirect ELISA

Cat No. 82964-1-PBS

Clone No.230204B3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), Indirect ELISA

Synonyms

SLC22A12, 230204B3, OAT4L, OATL4, Organic anion transporter 4-like protein

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Product Information

82964-1-PBS targets URAT1 in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag27805

  Product name: Recombinant human URAT1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 4-351 aa of BC053348

  Sequence: SELLDLVGGLGRFQVLQTMALMVSIMWLCTQSMLENFSAAVPSHRCWAPLLDNSTAQASILGSLSPEALLAISIPPGPNQRPHQCRRFRQPQWQLLDPNATATSWSEADTEPCVDGWVYDRSIFTSTIVAKWNLVCDSHALKPMAQSIYLAGILVGAAACGPASDRFGRRLVLTWSYLQMAVMGTAAAFAPAFPVYCLFRFLLAFAVAGVMMNTGTLLMEWTAARARPLVMTLNSLGFSFGHGLTAAVAYGVRDWTLLQLVVSVPFFLCFLYSWWLAESARWLLTTGRLDWGLQELWRVAAINGKGAVQDTLTPEVLLSAMREELSMGQPPASLGTLLRMPGLRFRTC

• Full Name: solute carrier family 22 (organic anion/urate transporter), member 12
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC053348
• Gene Symbol: URAT1
• Gene ID (NCBI): 116085
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q96S37
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Human urate anion exchanger (URAT1), also known as SLC22A12, is a urate transporter located in the proximal tubules of human kidney and responsible for urate reabsorption. Defects in gene encoding URAT1 cause hypouricemia renal type 1 (RHUC1). This antibody recognizes the glycosylated URAT1 at 70 kDa.