Tested Applications
| Positive WB detected in | HepG2 cells, HeLa cells, mouse liver tissue, mouse kidney tissue, rat kidney tissue, human kidney tissue |
| Positive FC (Intra) detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
82964-1-RR targets URAT1 in WB, IF, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag27805 Product name: Recombinant human URAT1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 4-351 aa of BC053348 Sequence: SELLDLVGGLGRFQVLQTMALMVSIMWLCTQSMLENFSAAVPSHRCWAPLLDNSTAQASILGSLSPEALLAISIPPGPNQRPHQCRRFRQPQWQLLDPNATATSWSEADTEPCVDGWVYDRSIFTSTIVAKWNLVCDSHALKPMAQSIYLAGILVGAAACGPASDRFGRRLVLTWSYLQMAVMGTAAAFAPAFPVYCLFRFLLAFAVAGVMMNTGTLLMEWTAARARPLVMTLNSLGFSFGHGLTAAVAYGVRDWTLLQLVVSVPFFLCFLYSWWLAESARWLLTTGRLDWGLQELWRVAAINGKGAVQDTLTPEVLLSAMREELSMGQPPASLGTLLRMPGLRFRTC Predict reactive species |
| Full Name | solute carrier family 22 (organic anion/urate transporter), member 12 |
| Calculated Molecular Weight | 60 kDa |
| Observed Molecular Weight | 70 kDa |
| GenBank Accession Number | BC053348 |
| Gene Symbol | URAT1 |
| Gene ID (NCBI) | 116085 |
| RRID | AB_3670710 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q96S37 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Human urate anion exchanger (URAT1), also known as SLC22A12, is a urate transporter located in the proximal tubules of human kidney and responsible for urate reabsorption. Defects in gene encoding URAT1 cause hypouricemia renal type 1 (RHUC1). This antibody recognizes the glycosylated URAT1 at 70 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for URAT1 antibody 82964-1-RR | Download protocol |
| WB protocol for URAT1 antibody 82964-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The URAT1 Recombinant monoclonal antibody (230204B3), catalog number 82964-1-RR, has a total of 2 citations. These citations appear in Phytomedicine (IF 11.3) and J Pain Res (IF 3.1). The associated research fields include uric acid metabolism, chronic kidney disease, and gouty arthritis with hyperuricemia, focusing on natural compound identification and mechanistic studies of traditional formulas in rat models.
| Species | Application | Title |
|---|---|---|
Phytomedicine Identifying the natural compounds in Jinqian Xuduan Decoction for simultaneous uric acid lowering and chronic kidney disease amelioration | ||
J Pain Res Mechanistic Study on the Therapeutic Effects of Juanbi Lijieqing Formula in a Rat Model of Gouty Arthritis with Hyperuricemia |
