USP14 Polyclonal antibody

• Featured Product
• KD/KO Validated

USP14 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

19

Cat no : 14517-1-AP

Synonyms

Ubiquitin thiolesterase 14, Ubiquitin thioesterase 14, Ubiquitin carboxyl-terminal hydrolase 14, TGT, EC:3.4.19.12

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Validation Data Gallery

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  WB analysis using 14517-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14517-1-AP (USP14 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 14517-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14517-1-AP (USP14 antibody) at dilution of 1:9000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14517-1-AP

  WB result of USP14 antibody (14517-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP14 transfected HeLa cells.

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  WB analysis using 14517-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14517-1-AP (USP14 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 14517-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 14517-1-AP (USP14 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14517-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14517-1-AP (USP14 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 14517-1-AP

  IP result of anti-USP14 (IP:14517-1-AP, 4ug; Detection:14517-1-AP 1:1000) with mouse liver tissue lysate 4000ug.

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  IF Staining of HepG2 using 14517-1-AP

  Immunofluorescent analysis of HepG2 cells, using USP14 antibody 14517-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: DU 145 cells, HeLa cells, mouse heart tissue, HEK-293T cells, HEK-293 cells, SKOV-3 cells, Neuro-2a cells
• Positive IP detected in: mouse liver tissue
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:18000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 5 publications below
• WB: See 17 publications below
• IHC: See 4 publications below
• IF: See 4 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

14517-1-AP targets USP14 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: USP14 fusion protein Ag5979
• Full Name: ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase)
• Calculated Molecular Weight: 54 kDa
• Observed Molecular Weight: 52-60 kDa
• GenBank Accession Number: BC003556
• Gene Symbol: USP14
• Gene ID (NCBI): 9097
• RRID: AB_2257124
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

USP14(Ubiquitin carboxyl-terminal hydrolase 14) is also named as TGT and belongs to the peptidase C19 family. Mammalian USP14 is unique among known UBP enzymes in that it is activated catalytically upon specific association with the 26S proteasome. USP14 inhibition accelerated the degradation of oxidized proteins and enhanced resistance to oxidative stress.This protein has a 45-kDa catalytic domain (PMID:16211010).

Publications

Species	Application	Title
human	WB,IF	Autophagy; USP14 regulates DNA damage repair by targeting RNF168-dependent ubiquitination.; Authors - Arishya Sharma
pig	WB,IF,IP	Autophagy; Inhibition of USP14 influences alphaherpesvirus proliferation by degrading viral VP16 protein via ER stress-triggered selective autophagy.; Authors - Sheng-Li Ming ; KD Validated
human	IF	Cell Prolif; SPAG5-AS1 inhibited autophagy and aggravated apoptosis of podocytes via SPAG5/AKT/mTOR pathway.; Authors - Jun Xu
human	WB	Front Pharmacol; SOAT1 Promotes Gastric Cancer Lymph Node Metastasis Through Lipid Synthesis.; Authors - Tingting Zhu
human	IHC	J Biol Chem; The USP14-NLRC5 pathway inhibits titanium particle-induced osteolysis in mice by suppressing NF-κB and PI3K/AKT activities.; Authors - Guibin Fang
human,mouse	WB,CoIP	Oral Dis; USP14 promotes the cancer stem-like cell properties of OSCC via promoting SOX2 deubiquitination; Authors - Chang Liu ; KD Validated