Tested Applications
| Positive WB detected in | Daudi cells, HepG2 cells, hTERT-RPE1 cells, mouse kidney tissue, rat kidney tissue |
| Positive IHC detected in | human colon cancer tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, A549 cells, HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:800-1:3200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15402-1-AP targets USP30 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7620 Product name: Recombinant human USP30 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 159-508 aa of BC004868 Sequence: VITSSLEDERDRQPRVTHLFDVHSLEQQSEITPKQITCRTRGSPHPTSNHWKSQHPFHGRLTSNMVCKHCEHQSPVRFDTFDSLSLSIPAATWGHPLTLDHCLHHFISSESVRDVVCDNCTKIEAKGTLNGEKVEHQRTTFVKQLKLGKLPQCLCIHLQRLSWSSHGTPLKRHEHVQFNEFLMMDIYKYHLLGHKPSQHNPKLNKNPGPTLELQDGPGAPTPVLNQPGAPKTQIFMNGACSPSLLPTLSAPMPFPLPVVPDYSSSTYLFRLMAVVVHHGDMHSGHFVTYRRSPPSARNPLSTSNQWLWVSDDTVRKASLQEVLSSSAYLLFYERVLSRMQHQSQECKSEE Predict reactive species |
| Full Name | ubiquitin specific peptidase 30 |
| Calculated Molecular Weight | 58.5 kDa |
| Observed Molecular Weight | 59-67 kDa |
| GenBank Accession Number | BC004868 |
| Gene Symbol | USP30 |
| Gene ID (NCBI) | 84749 |
| RRID | AB_3085460 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q70CQ3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
USP30, a mitochondrion-localized deubiquitylase, regulates mitophagy. Mitochondrial damage stimulates parkin to assemble Lys 6, Lys 11 and Lys 63 chains on mitochondria, and that USP30 is a ubiquitin-specific deubiquitylase with a strong preference for cleaving Lys 6- and Lys 11-linked multimers. USP30 can be ubiquitinated by parkin (PARK2) at Lys-235 and Lys-289. USP30 inhibition is potentially beneficial for treating Parkinson disease by promoting mitochondrial clearance and quality control. USP30 was detected 67 kDa by ubiquitination (PMID: 34704599).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for USP30 antibody 15402-1-AP | Download protocol |
| IHC protocol for USP30 antibody 15402-1-AP | Download protocol |
| WB protocol for USP30 antibody 15402-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The USP30 Polyclonal antibody (Cat# 15402-1-AP) has 7 citations published in Nature, Materials Today Bio, BBA Molecular Basis of Disease, Experimental Neurology, Journal of Biochemical and Molecular Toxicology, Biochemical and Biophysical Research Communications, and Neuroreport. Research fields span tumor immune evasion, wound healing, ovarian cancer metastasis, Alzheimer's disease, breast cancer tamoxifen resistance, traumatic brain injury, and subarachnoid hemorrhage, with applications in WB, IHC, IF, and CoIP.
| Species | Application | Title |
|---|---|---|
Mater Today Bio Extracellular biogenic nanoscale mitochondria reprogram the wound microenvironment via ROS scavenging independent of cellular uptake. | ||
Biochim Biophys Acta Mol Basis Dis FOXF1 promotes ovarian cancer metastasis by facilitating HMGA2-mediated USP30-dependent S100A6 deubiquitination | ||
Exp Neurol Hyperbaric oxygen therapy ameliorates Alzheimer's disease pathology by restoration of mitophagy and suppressing neuroinflammation in 5xFAD mice. | ||
J Biochem Mol Toxicol USP30 Aggravating the Malignant Progression of Breast Cancer and Its Resistance to Tamoxifen by Inhibiting the Ubiquitination of TOMM40 | ||
Biochem Biophys Res Commun USP30 impairs mitochondrial quality control and aggravates oxidative damage after traumatic brain injury |
