USP39 Polyclonal antibody, PBS Only

• Home
• Products
• Primary Antibodies
• USP39 Polyclonal antibody, PBS Only (23865-1-PBS)

• 

  WB analysis using 23865-1-AP (same clone as 23865-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 23865-1-AP (USP39 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23865-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 23865-1-AP (same clone as 23865-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 23865-1-AP (USP39 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23865-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 23865-1-AP (same clone as 23865-1-PBS)

  WB result of USP39 antibody (23865-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP39 transfected HEK-293 cells. This data was developed using the same antibody clone with 23865-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 23865-1-AP (same clone as 23865-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 23865-1-AP (USP39 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23865-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 23865-1-AP (same clone as 23865-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 23865-1-AP (USP39 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23865-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 23865-1-AP (same clone as 23865-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 23865-1-AP (USP39 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23865-1-PBS in a different storage buffer formulation.

• 

  IP experiment of K-562 using 23865-1-AP (same clone as 23865-1-PBS)

  IP result of anti-USP39 (IP:23865-1-AP, 4ug; Detection:23865-1-AP 1:1000) with K-562 cells lysate 3200ug. This data was developed using the same antibody clone with 23865-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 23865-1-AP (same clone as 23865-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 23865-1-AP (USP39 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23865-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 23865-1-AP (same clone as 23865-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 23865-1-AP (USP39 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23865-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 23865-1-AP (same clone as 23865-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 23865-1-AP (USP39 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23865-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A431 using 23865-1-AP (same clone as 23865-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using USP39 antibody (23865-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 23865-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A549 using 23865-1-AP (same clone as 23865-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using USP39 antibody (23865-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 23865-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 23865-1-AP (same clone as 23865-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using USP39 antibody (23865-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 23865-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

USP39 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 23865-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

CGI 21, CGI21, CGI-21, EC:3.4.19.12, HSPC332

---

Product Information

23865-1-PBS targets USP39 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag20894

  Product name: Recombinant human USP39 protein

  Source: e coli.-derived, PET30a

  Tag: 6*His

  Domain: 1-357 aa of BC067273

  Sequence: MSGRSKRESRGSTRGKRESESRGSSGRVKRERDREREPEAASSRGSPVRVKREFEPASAREAPASVVPFVRVKREREVDEDSEPEREVRAKNGRVDSEDRRSRHCPYLDTINRSVLDFDFEKLCSISLSHINAYACLVCGKYFQGRGLKSHAYIHSVQFSHHVFLNLHTLKFYCLPDNYEIIDSSLEDITYVLKPTFTKQQIANLDKQAKLSRAYDGTTYLPGIVGLNNIKANDYANAVLQALSNVPPLRNYFLEEDNYKNIKRPPGDIMFLLVQRFGELMRKLWNPRNFKAHVSPHEMLQAVVLCSKKTFQITKQGDGVDFLSWFLNALHSALGGTKKKKKTIVTDVFQGSMRIFT

• Full Name: ubiquitin specific peptidase 39
• Calculated Molecular Weight: 565 aa, 65 kDa
• Observed Molecular Weight: 60~65 kDa
• GenBank Accession Number: BC067273
• Gene Symbol: USP39
• Gene ID (NCBI): 10713
• RRID: AB_2879343
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q53GS9
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

USP39 plays a role in many cellular processes including cellular antiviral response, epithelial morphogenesis, DNA repair or B-cell development. USP39 regulates apoptosis and G2/M cell cycle checkpoint in response to DNA damage by deubiquitinating and stabilizing CHK2