USP9X Polyclonal antibody

• Featured Product
• KD/KO Validated

USP9X Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

36

Reviews (1)

Cat no : 55054-1-AP

Synonyms

Fat facets protein-related, X-linked, FAM, FAF, EC:3.4.19.12, DFFRX

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Validation Data Gallery

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  WB analysis using 55054-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55054-1-AP (USP9X antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 55054-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55054-1-AP (USP9X antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 55054-1-AP

  IP result of anti-USP9X (IP:55054-1-AP, 4ug; Detection:55054-1-AP 1:1000) with HeLa cells lysate 1280 ug.

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  IP experiment of HeLa using 55054-1-AP

  IP result of anti-USP9X (IP:55054-1-AP, 4ug; Detection:55054-1-AP 1:4000) with HeLa cells lysate 2000 ug.

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  IHC staining of human pancreas cancer using 55054-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 55054-1-AP (USP9X antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 55054-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 55054-1-AP (USP9X antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 55054-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using USP9X antibody (55054-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HeLa using 55054-1-AP

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug USP9X Polyclonal antibody (55054-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, K-562 cells, mouse brain tissue, rat brain tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 17 publications below
• WB: See 31 publications below
• IHC: See 4 publications below
• IF: See 6 publications below
• IP: See 5 publications below
• CoIP: See 5 publications below

Product Information

55054-1-AP targets USP9X in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: ubiquitin specific peptidase 9, X-linked
• Calculated Molecular Weight: 292 kDa
• Observed Molecular Weight: 260-290 kDa
• GenBank Accession Number: NM_001039590
• Gene Symbol: USP9X
• Gene ID (NCBI): 8239
• RRID: AB_10792932
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

USP9X, also named as DFFRX, FAM and USP9, belongs to the peptidase C19 family. It may function as a ubiquitin-protein or polyubiquitin hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. It plays an important role regulatory role at the level of protein turnover by preventing degradation of proteins through the removal of conjugated ubiquitin. USP9X is an essential component of TGF-beta/BMP signaling cascade. Deubiqitination of SMAD4 by USP9X restores its competence to mediate TGF-beta signaling. USP9X regulates chromosome alignment and segregation in mitosis by regulating the localization of BIRC5/survivin to mitotic centromeres. The antibody is specific to USP9X. It has no cross reaction to USP9Y.

Publications

Species	Application	Title
human	WB	Nat Commun; Pharmaceutical targeting of OTUB2 sensitizes tumors to cytotoxic T cells via degradation of PD-L1; Authors - Wenfeng Ren
human	WB	Nat Commun; BioE3 identifies specific substrates of ubiquitin E3 ligases; Authors - Orhi Barroso-Gomila
human	WB	Nat Commun; Integrative oncogene-dependency mapping identifies RIT1 vulnerabilities and synergies in lung cancer.; Authors - Athea Vichas ; KO Validated
human	IF	Nat Commun; Antagonistic activities of CDC14B and CDK1 on USP9X regulate WT1-dependent mitotic transcription and survival.; Authors - Michael Dietachmayr
human	WB,IF,IP	Nat Commun; USP9X regulates centrosome duplication and promotes breast carcinogenesis.; Authors - Xin Li ; KO Validated
human	WB	Cell Death Differ; USP9X-mediated KDM4C deubiquitination promotes lung cancer radioresistance by epigenetically inducing TGF-β2 transcription.; Authors - Xiaohua Jie

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Echo (Verified Customer) (03-20-2024)

The antibody quality was pretty good. Band was clear.

• Applications: Western Blot
• Primary Antibody Dilution: 1:10000
• Cell Tissue Type: HEK293T

USP9X Antibody Western Blot validation (1:10000 dilution) in HEK293T (Cat no:55054-1-AP)