CoraLite® Plus 488-conjugated UTP18 Recombinant antibody

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  IF Staining of A431 using CL488-84035-3

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CoraLite® Plus 488 UTP18 antibody (CL488-84035-3, Clone: 241240F1 ) at dilution of 1:200, (CL594-phalloidin, red).

UTP18 Recombinant Antibody for IF/ICC

Cat No. CL488-84035-3

Clone No.241240F1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Synonyms

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Tested Applications

• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-84035-3 targets UTP18 in IF/ICC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: UTP18 fusion protein Ag34662
• Full Name: UTP18, small subunit (SSU) processome component, homolog (yeast)
• Calculated Molecular Weight: 62 kDa
• Observed Molecular Weight: 62 kDa
• GenBank Accession Number: NM_016001
• Gene Symbol: UTP18
• Gene ID (NCBI): 51096
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9Y5J1
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

UTP18 (UTP18 small subunit processome component), also known as WDR50. It is expected to be located in nucleus. The protein is one part of the small subunit (SSU) processome, first precursor of the small eukaryotic ribosomal subunit. During the assembly of the SSU processome in the nucleolus, many ribosome biogenesis factors, an RNA chaperone and ribosomal proteins associate with the nascent pre-rRNA and work in concert to generate RNA folding, modifications, rearrangements and cleavage as well as targeted degradation of pre-ribosomal RNA by the RNA exosome. Involved in nucleolar processing of pre-18S ribosomal RNA. The calculated molecular weight of UTP18 is 62 kDa.