Uteroglobin/CC10 Polyclonal antibody, PBS Only

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• Uteroglobin/CC10 Polyclonal antibody, PBS Only (26909-1-PBS)

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  WB analysis of rat lung using 26909-1-AP (same clone as 26909-1-PBS)

  rat lung tissue were subjected to SDS PAGE followed by western blot with 26909-1-AP (Uteroglobin Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26909-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung using 26909-1-AP (same clone as 26909-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 26909-1-AP (Uteroglobin Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26909-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung using 26909-1-AP (same clone as 26909-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 26909-1-AP (Uteroglobin Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26909-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse lung using 26909-1-AP (same clone as 26909-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 26909-1-AP (Uteroglobin/CC10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26909-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse lung using 26909-1-AP (same clone as 26909-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 26909-1-AP (Uteroglobin/CC10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26909-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 26909-1-AP (same clone as 26909-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol) fixed A549 cells using 26909-1-AP (Uteroglobin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 26909-1-PBS in a different storage buffer formulation.

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  FC experiment of A549 using 26909-1-AP (same clone as 26909-1-PBS)

  1X10^6 A549 cells were intracellularly stained with 0.4 ug Anti-Human Uteroglobin/CC10 (26909-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 26909-1-PBS in a different storage buffer formulation.

Uteroglobin/CC10 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Cat No. 26909-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

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Product Information

26909-1-PBS targets Uteroglobin/CC10 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag25530

  Product name: Recombinant human Uteroglobin protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 22-91 aa of BC004481

  Sequence: EICPSFQRVIETLLMDTPSSYEAAMELFSPDQDMREAGAQLKKLVDTLPQKPRESIIKLMEKIAQSSLCN

• Full Name: secretoglobin, family 1A, member 1 (uteroglobin)
• Calculated Molecular Weight: 10 kDa
• Observed Molecular Weight: 9 kDa
• GenBank Accession Number: BC004481
• Gene Symbol: Uteroglobin
• Gene ID (NCBI): 7356
• RRID: AB_2880680
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P11684
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Uteroglobin, also known as CC10, is a member of the secretoglobin family of small secreted proteins. It is produced by the nonciliated, nonmucous, secretory epithelial clara cells of the pulmonary airways, was first identified in lung lavage by Singh and colleagues. It has been implicated in numerous functions including anti-inflammation, inhibition of phospholipase A2 and the sequestering of hydrophobic ligands. Defects in this gene are associated with a susceptibility to asthma.