VAMP4 Monoclonal antibody

VAMP4 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Pig, Rat, Mouse and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

3A6F2

Cat no : 67219-1-Ig

Synonyms

VAMP 4, VAMP24, VAMP4

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Validation Data Gallery

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  WB analysis using 67219-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67219-1-Ig (VAMP4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 67219-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67219-1-Ig (VAMP4 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 67219-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67219-1-Ig (VAMP4 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HEK-293 using 67219-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 67219-1-Ig (VAMP4 antibody), at dilution of 1:300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, Jurkat cells, K-562 cells, pig brain tissue, rat brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:150-1:600
• Immunofluorescence (IF): IF : 1:150-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

67219-1-Ig targets VAMP4 in WB, IF, IHC, ELISA applications and shows reactivity with Human, Pig, Rat, Mouse samples.

• Tested Reactivity: Human, Pig, Rat, Mouse
• Cited Reactivity: mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: VAMP4 fusion protein Ag27854
• Full Name: vesicle-associated membrane protein 4
• Calculated Molecular Weight: 16 kDa
• Observed Molecular Weight: 18 kDa
• GenBank Accession Number: BC007019
• Gene Symbol: VAMP4
• Gene ID (NCBI): 8674
• RRID: AB_2882510
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VAMP4 is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family and the SNARE superfamily. Characterized by a common sequence called the SNARE motif, SNARE proteins are involved in membrane fusion and vesicular transport (PMID: 11252968). VAMP4 may play a role in trans-Golgi network to endosome transport.

Publications

Species	Application	Title
mouse	IF	eNeuro; ADP ribosylation factor 4 (Arf4) regulates radial migration through N-cadherin trafficking during cerebral cortical development; Authors - Yoshinobu Hara