VAMP8 Recombinant antibody

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  WB analysis using 85400-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85400-4-RR (VAMP8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 85400-4-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 85400-4-RR (VAMP8 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 85400-4-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 85400-4-RR (VAMP8 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of THP-1 using 85400-4-RR

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using VAMP8 antibody (85400-4-RR, Clone: 242830B11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 85400-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85400-4-RR against Human VAMP8 were performed. The affinity constant is 0.631 nM.

VAMP8 Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 85400-4-RR

Clone No.242830B11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

VAMP-8, 242830B11, EDB, Endobrevin, VAMP 8

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Tested Applications

• Positive WB detected in: THP-1 cells, HL-60 cells, A431 cells, HeLa cells, rat kidney tissue, mouse kidney tissue
• Positive IHC detected in: human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: THP-1 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85400-4-RR targets VAMP8 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: VAMP8 fusion protein Ag7903
• Full Name: vesicle-associated membrane protein 8 (endobrevin)
• Calculated Molecular Weight: 11 kDa
• Observed Molecular Weight: 15 kDa
• GenBank Accession Number: BC001634
• Gene Symbol: VAMP8
• Gene ID (NCBI): 8673
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9BV40
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VAMP8, also named as endobrevin, is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family and the SNARE (soluble NSF-attachment protein receptor) superfamily. Characterized by a common sequence called the SNARE motif, SNARE proteins are involved in membrane fusion and vesicular transport (PMID: 11252968). VAMP8 is involved in autophagy through the direct control of autophagosome membrane fusion with the lysososome membrane. It is required for dense-granule secretion in platelets and plays a role in regulated enzyme secretion in pancreatic acinar cells. VAMP8 is also involved in the abscission of the midbody during cell division, which leads to completely separate daughter cells.