CoraLite® Plus 488-conjugated VAMP8 Recombinant monoclonal antibody

• 

  IF Staining of HeLa using CL488-85400-4

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 VAMP8 antibody (CL488-85400-4, Clone: 242830B11 ) at dilution of 1:200.

• 

  IF Staining of HEK-293 using CL488-85400-4

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CoraLite® Plus 488 VAMP8 antibody (CL488-85400-4, Clone: 242830B11 ) at dilution of 1:200.

VAMP8 Uni-rAb^® Recombinant Antibody for IF/ICC

Cat No. CL488-85400-4

Clone No.242830B11

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Synonyms

VAMP-8, 242830B11, EDB, Endobrevin, VAMP 8

---

Tested Applications

• Positive IF/ICC detected in: HeLa cells, HEK-293 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-85400-4 targets VAMP8 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag7903

  Product name: Recombinant human VAMP8 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-81 aa of BC001634

  Sequence: MEEASEGGGNDRVRNLQSEVEGVKNIMTQNVERILARGENLEHLRNKTEDLEATSEHFKTTSQKVARKFWWKNVKMIVLIC

• Full Name: vesicle-associated membrane protein 8 (endobrevin)
• Calculated Molecular Weight: 11 kDa
• Observed Molecular Weight: 15 kDa
• GenBank Accession Number: BC001634
• Gene Symbol: VAMP8
• Gene ID (NCBI): 8673
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9BV40
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

VAMP8, also named as endobrevin, is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family and the SNARE (soluble NSF-attachment protein receptor) superfamily. Characterized by a common sequence called the SNARE motif, SNARE proteins are involved in membrane fusion and vesicular transport (PMID: 11252968). VAMP8 is involved in autophagy through the direct control of autophagosome membrane fusion with the lysososome membrane. It is required for dense-granule secretion in platelets and plays a role in regulated enzyme secretion in pancreatic acinar cells. VAMP8 is also involved in the abscission of the midbody during cell division, which leads to completely separate daughter cells.