VAPA Monoclonal antibody

VAPA Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

1F10F9

Cat no : 67845-1-Ig

Synonyms

33 kDa VAMP associated protein, hVAP 33, VAMP A, VAMP associated protein A, VAP 33, VAP A, VAP33, VAPA



Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, Neuro-2a cells
Positive IHC detected inhuman liver cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

67845-1-Ig targets VAPA in WB, IHC,ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen VAPA fusion protein Ag7819
Full Name VAMP (vesicle-associated membrane protein)-associated protein A, 33kDa
Calculated molecular weight 28 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC002992
Gene symbol VAPA
Gene ID (NCBI) 9218
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

VAPA (Vesicle-associated membrane protein-associated protein A), also known as VAP33, is a 33-kDa, ubiquitously expressed integral membrane protein of the VAMP-associated protein (VAP) family. It is present in the plasma membrane and in intracellular vesicles, playing a role in vesicle trafficking.

Protocols

Product Specific Protocols
WB protocol for VAPA antibody 67845-1-IgDownload protocol
IHC protocol for VAPA antibody 67845-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols