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VAPB Polyclonal antibody
VAPB Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 14477-1-AP
Validation Data Gallery
|Positive WB detected in||HeLa cells, mouse skeletal muscle tissue, HepG2 cells, rat brain tissue, Jurkat cells, mouse brain tissue, mouse liver tissue, rat liver tissue|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human breast cancer tissue, mouse liver tissue, human pancreas cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:2000-1:12000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
14477-1-AP targets VAPB in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, monkey|
|Host / Isotype||Rabbit / IgG|
|Immunogen||VAPB fusion protein Ag5857|
|Full Name||VAMP (vesicle-associated membrane protein)-associated protein B and C|
|Calculated molecular weight||27 kDa|
|Observed molecular weight||27 kDa|
|GenBank accession number||BC001712|
|Gene ID (NCBI)||9217|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Vesicle-associated membrane protein-associated protein B (VAPB) is an integral membrane protein localized to the endoplasmic reticulum (ER) membrane. VAPB has been implicated in various cellular processes, including ER stress, the unfolded protein response (UPR) and calcium homeostasis regulation. The mutations in the gene of VAPB cause amyotrophic lateral sclerosis 8 (ALS8) and some other related forms of motor neuron disease including late onset spinal muscular atrophy.
|Product Specific Protocols|
|WB protocol for VAPB antibody 14477-1-AP||Download protocol|
|IHC protocol for VAPB antibody 14477-1-AP||Download protocol|
|IF protocol for VAPB antibody 14477-1-AP||Download protocol|
|IP protocol for VAPB antibody 14477-1-AP||Download protocol|
|FC protocol for VAPB antibody 14477-1-AP||Download protocol|
|Click here to view our Standard Protocols|
A High-Density Human Mitochondrial Proximity Interaction Network.
Nat Cell Biol
MIROs and DRP1 drive mitochondrial-derived vesicle biogenesis and promote quality control.
DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling.
MIGA2 Links Mitochondria, the ER, and Lipid Droplets and Promotes De Novo Lipogenesis in Adipocytes.
J Clin Invest
SPTLC1 variants associated with ALS produce distinct sphingolipid signatures through impaired interaction with ORMDL proteins.
Proteomic mapping and targeting of mitotic pericentriolar material in tumors bearing centrosome amplification.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Wei (Verified Customer) (09-22-2020)
The antibody have predicted band on western blot.