Tested Applications
| Positive WB detected in | C6 cells, Jurkat cells, MCF-7 cells, SH-SY5Y cells, mouse brain tissue, rat brain tissue |
| Positive IHC detected in | mouse brain tissue, human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
22016-1-AP targets VAT1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag16652 Product name: Recombinant human VAT1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-393 aa of BC015041 Sequence: MSDEREVAEAATGEDASSPPPKTEAASDPQHPAASEGAAAAAASPPLLRCLVLTGFGGYDKVKLQSRPAAPPAPGPGQLTLRLRACGLNFADLMARQGLYDRLPPLPVTPGMEGAGVVIAVGEGVSDRKAGDRVMVLNRSGMWQEEVTVPSVQTFLIPEAMTFEEAAALLVNYITAYMVLFDFGNLQPGHSVLVHMAAGGVGMAAVQLCRTVENVTVFGTASASKHEALKENGVTHPIDYHTTDYVDEIKKISPKGVDIVMDPLGGSDTAKGYNLLKPMGKVVTYGMANLLTGPKRNLMALARTWWNQFSVTALQLLQANRAVCGFHLGYLDGEVELVSGVVARLLALYNQGHIKPHIDSVWPFEKVADAMKQMQEKKNVGKVLLVPGPEKEN Predict reactive species |
| Full Name | vesicle amine transport protein 1 homolog (T. californica) |
| Calculated Molecular Weight | 42 kDa |
| Observed Molecular Weight | 42-50 kDa |
| GenBank Accession Number | BC015041 |
| Gene Symbol | VAT1 |
| Gene ID (NCBI) | 10493 |
| RRID | AB_11182282 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q99536 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for VAT1 antibody 22016-1-AP | Download protocol |
| WB protocol for VAT1 antibody 22016-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Anti-VAT1 antibody (Cat# 22016-1-AP) has 2 citations. These publications appear in Cancer Immunol Immunother and Oncol Rep. The research focuses on glioma and glioblastoma, specifically examining VAT1's role in immunosuppressive mechanisms of diffuse gliomas and its value as a prognostic biomarker predicting malignancy in glioblastoma. Both studies utilized the antibody for WB and IHC applications in human samples.
| Species | Application | Title |
|---|---|---|
Cancer Immunol Immunother Novel roles of VAT1 expression in the immunosuppressive action of diffuse gliomas.
| ||
Oncol Rep High expression of VAT1 is a prognostic biomarker and predicts malignancy in glioblastoma. |
Reviews
What customers say
The single review reports a 5-star rating for Western Blot on HEK293T cells. The antibody performed well at a 1:2000 dilution in 5% milk in BSA, with overnight incubation at 4°C. Overall, the reviewer found the product effective and reliable for their application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Sarah (Verified Customer) (09-05-2025) | Antibody worked well for western blot. Used with 1:2000 dilution in 5% milk in BSA, with overnight incubation at 4C.
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