VAT1 Recombinant monoclonal antibody, PBS Only

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• VAT1 Recombinant monoclonal antibody, PBS Only (86847-1-PBS)

• 

  WB analysis using 86847-1-RR (same clone as 86847-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86847-1-RR (VAT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86847-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 86847-1-RR (same clone as 86847-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86847-1-RR (VAT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86847-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 86847-1-RR (same clone as 86847-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 86847-1-RR (VAT1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86847-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 86847-1-RR (same clone as 86847-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 86847-1-RR (VAT1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86847-1-PBS in a different storage buffer formulation.

• 

  IF Staining of SH-SY5Y using 86847-1-RR (same clone as 86847-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using VAT1 antibody (86847-1-RR, Clone: 251841D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86847-1-PBS in a different storage buffer formulation.

VAT1 Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 86847-1-PBS

Clone No.251841D6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

EC:1.-.-.-, Synaptic vesicle membrane protein VAT-1 homolog, VATI

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Product Information

86847-1-PBS targets VAT1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag16652

  Product name: Recombinant human VAT1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-393 aa of BC015041

  Sequence: MSDEREVAEAATGEDASSPPPKTEAASDPQHPAASEGAAAAAASPPLLRCLVLTGFGGYDKVKLQSRPAAPPAPGPGQLTLRLRACGLNFADLMARQGLYDRLPPLPVTPGMEGAGVVIAVGEGVSDRKAGDRVMVLNRSGMWQEEVTVPSVQTFLIPEAMTFEEAAALLVNYITAYMVLFDFGNLQPGHSVLVHMAAGGVGMAAVQLCRTVENVTVFGTASASKHEALKENGVTHPIDYHTTDYVDEIKKISPKGVDIVMDPLGGSDTAKGYNLLKPMGKVVTYGMANLLTGPKRNLMALARTWWNQFSVTALQLLQANRAVCGFHLGYLDGEVELVSGVVARLLALYNQGHIKPHIDSVWPFEKVADAMKQMQEKKNVGKVLLVPGPEKEN

• Full Name: vesicle amine transport protein 1 homolog (T. californica)
• Calculated Molecular Weight: 42 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC015041
• Gene Symbol: VAT1
• Gene ID (NCBI): 10493
• RRID: AB_3745159
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q99536
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Vesicle amine transport protein 1 (VAT1) is expressed in neurons and neuroendocrine cells, where it participates in vesicular monoamine uptake and in the docking/fusion steps of neurotransmitter release. Beyond the nervous system, VAT1 binds anionic phospholipids (phosphatidic acid and phosphatidylserine) and catalyses their inter-membrane transfer, thereby integrating lipid metabolism with vesicle trafficking. Recent tumour-biology studies have revealed that VAT1 is frequently up-regulated in aggressive gliomas and gastric cancers; its abundance correlates with enhanced cell migration, radioresistance and an immunosuppressive micro-environment. (PMID: 39832459,PMID: 31524269)