VAT1 Recombinant monoclonal antibody

• Home
• Products
• Primary Antibodies
• Recombinant Antibodies
• VAT1 Recombinant monoclonal antibody (86847-1-RR)

• 

  WB analysis using 86847-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86847-1-RR (VAT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 86847-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86847-1-RR (VAT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 86847-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 86847-1-RR (VAT1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 86847-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 86847-1-RR (VAT1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of SH-SY5Y using 86847-1-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using VAT1 antibody (86847-1-RR, Clone: 251841D6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

VAT1 Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 86847-1-RR

Clone No.251841D6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

EC:1.-.-.-, Synaptic vesicle membrane protein VAT-1 homolog, VATI

---

Tested Applications

• Positive WB detected in: SH-SY5Y cells, NIH/3T3 cells, C6 cells, mouse brain tissue, rat brain tissue, Jurkat cells, MCF-7 cells
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86847-1-RR targets VAT1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag16652

  Product name: Recombinant human VAT1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-393 aa of BC015041

  Sequence: MSDEREVAEAATGEDASSPPPKTEAASDPQHPAASEGAAAAAASPPLLRCLVLTGFGGYDKVKLQSRPAAPPAPGPGQLTLRLRACGLNFADLMARQGLYDRLPPLPVTPGMEGAGVVIAVGEGVSDRKAGDRVMVLNRSGMWQEEVTVPSVQTFLIPEAMTFEEAAALLVNYITAYMVLFDFGNLQPGHSVLVHMAAGGVGMAAVQLCRTVENVTVFGTASASKHEALKENGVTHPIDYHTTDYVDEIKKISPKGVDIVMDPLGGSDTAKGYNLLKPMGKVVTYGMANLLTGPKRNLMALARTWWNQFSVTALQLLQANRAVCGFHLGYLDGEVELVSGVVARLLALYNQGHIKPHIDSVWPFEKVADAMKQMQEKKNVGKVLLVPGPEKEN

• Full Name: vesicle amine transport protein 1 homolog (T. californica)
• Calculated Molecular Weight: 42 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC015041
• Gene Symbol: VAT1
• Gene ID (NCBI): 10493
• RRID: AB_3745159
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q99536
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Vesicle amine transport protein 1 (VAT1) is expressed in neurons and neuroendocrine cells, where it participates in vesicular monoamine uptake and in the docking/fusion steps of neurotransmitter release. Beyond the nervous system, VAT1 binds anionic phospholipids (phosphatidic acid and phosphatidylserine) and catalyses their inter-membrane transfer, thereby integrating lipid metabolism with vesicle trafficking. Recent tumour-biology studies have revealed that VAT1 is frequently up-regulated in aggressive gliomas and gastric cancers; its abundance correlates with enhanced cell migration, radioresistance and an immunosuppressive micro-environment. (PMID: 39832459,PMID: 31524269)