VBP1 Polyclonal antibody

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• VBP1 Polyclonal antibody (14345-1-AP)

VBP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 14345-1-AP Copied!

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

PFD3, PFDN3, VBP-1, VHL-binding protein 1, von Hippel-Lindau-binding protein 1

• 

  WB analysis using 14345-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14345-1-AP (VBP1 antibody) at dilution of 1:8000 incubated at 4 degree celsius over night.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse liver using 14345-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14345-1-AP (VBP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of K-562 using 14345-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14345-1-AP (VBP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HepG2 using 14345-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14345-1-AP (VBP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human thyroid cancer using 14345-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 14345-1-AP (VBP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human thyroid cancer using 14345-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 14345-1-AP (VBP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HepG2 using 14345-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using VBP1 antibody (14345-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: C6 cells, K-562 cells, HepG2 cells, mouse liver tissue, NIH/3T3 cells, mouse brain tissue, rat brain tissue
• Positive IHC detected in: human thyroid cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:10000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14345-1-AP targets VBP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5685

  Product name: Recombinant human VBP1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-197 aa of BC046094

  Sequence: MAAVKDSCGKGEMATGNGRRLHLGIPEAVFVEDVDSFMKQPGNETADTVLKKLDEQYQKYKFMELNLAQKKRRLKGQIPEIKQTLEILKYMQKKKESTNSMETRFLLADNLYCKASVPPTDKVCLWLGANVMLEYDIDEAQALLEKNLSTATKNLDSLEEDLDFLRDQFTTTEVNMARVYNWDVKRRNKDDSTKNKA

• Full Name: von Hippel-Lindau binding protein 1
• Calculated Molecular Weight: 23 kDa
• Observed Molecular Weight: 23-25 kDa
• GenBank Accession Number: BC046094
• Gene Symbol: VBP1
• Gene ID (NCBI): 7411
• RRID: AB_2213910
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P61758
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The VBP1 Polyclonal antibody (Cat# 14345-1-AP) has 1 citation. It appears in PLoS Genetics (2020), in a study examining how the prefoldin complex stabilizes the von Hippel-Lindau protein against aggregation and degradation. The associated research field is protein folding, chaperone biology, and tumor suppressor regulation. The antibody was used for immunofluorescence in this publication.

Automatically generated from the 1 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
	IF	PLoS Genet; The prefoldin complex stabilizes the von Hippel-Lindau protein against aggregation and degradation.; Authors - Franck Chesnel