Mouse VCAM-1/CD106 Recombinant antibody, PBS Only (Detector)

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  Sandwich ELISA standard curve of MP00701-4

  Sandwich ELISA standard curve of MP00701-4, Mouse VCAM-1/CD106 Recombinant Matched Antibody Pair - PBS only. 83719-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0639. 83719-3-PBS was HRP conjugated as the detection antibody. Range: 46.88-3000 pg/mL.

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  Sample test of MP00701-4

  Serum of eight mice was measured. The VCAM-1 concentration of detected samples was determined to be 572.76 ng/mL with a range of 483.18-844.96 ng/mL.

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  Sample test of MP00701-4

  Urine of eight mice was measured. The VCAM-1 concentration of detected samples was determined to be 71.97 ng/mL with a range of 8.65-105.58 ng/mL.

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  Sample test of MP00701-4

  Mouse lung, cut into 1-2 mm pieces, was cultured for 3 days in 25-30 mL of RPMI supplemented with 10% fetal bovine serum and unstimulated or stimulated with 10 μg/mL ConA. The mean VCAM-1 concentration was determined to be 14.2 ng/mL in unstimulated mouse lung superantant, 20.9 ng/mL in ConA stimulated mouse lung superantant.

VCAM-1/CD106 Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 83719-3-PBS

Clone No.240597D2

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

CD106, Vcam1, VCAM-1, Vcam 1

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Product Information

83719-3-PBS targets VCAM-1/CD106 as part of a matched antibody pair:

MP00701-4: 83719-1-PBS capture and 83719-3-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: VCAM-1/CD106 fusion protein Eg0639
• Full Name: vascular cell adhesion molecule 1
• Calculated Molecular Weight: 81 kDa
• GenBank Accession Number: CAA47989.1
• Gene Symbol: VCAM-1
• Gene ID (NCBI): 22329
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P29533
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.