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Anti-Human VHL Rabbit Recombinant Antibody, PBS Only (98753-3-PBS)

Anti-Human VHL Rabbit Recombinant Antibody, PBS Only

Name: Anti-Human VHL Rabbit Recombinant Antibody, PBS Only, 1mg
Brand: Proteintech
SKU: 98753-3-PBS-1MG

VHL Uni-rAb^® Recombinant Antibody for FC (Intra)

Cat No. 98753-3-PBS

Clone No.251968A5

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Synonyms

Protein G7, pVHL, RCA1, VHL 1, VHL1

Formulation: PBS Only

PBS, Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

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Filter: AllFC (Intra)

FC experiment of Transfected CHO using 98753-3-RR (same clone as 98753-3-PBS)
1x10^6 CHO cells or VHL transfected CHO cells were intracellularly stained with 0.25 ug Anti-Human VHL Rabbit RecAb (98753-3-RR, Clone: 251968A5) or 0.25 ug Isotype Control, and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00017). This data was developed using the same antibody clone with 98753-3-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
1x10^6 CHO cells or VHL transfected CHO cells were intracellularly stained with 0.25 ug Anti-Human VHL Rabbit RecAb (98753-3-RR, Clone: 251968A5) or 0.25 ug Isotype Control, and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00017). This data was developed using the same antibody clone with 98753-3-PBS in a different storage buffer formulation.
FC experiment of Transfected CHO using 98753-3-RR (same clone as 98753-3-PBS)
x10^6 CHO cells or VHL transfected CHO cells were intracellularly stained with 0.25 ug Anti-Human VHL Rabbit RecAb (98753-3-RR, Clone:251968A5) (red) or 0.25 ug Isotype Control (blue), and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00017). This data was developed using the same antibody clone with 98753-3-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
x10^6 CHO cells or VHL transfected CHO cells were intracellularly stained with 0.25 ug Anti-Human VHL Rabbit RecAb (98753-3-RR, Clone:251968A5) (red) or 0.25 ug Isotype Control (blue), and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00017). This data was developed using the same antibody clone with 98753-3-PBS in a different storage buffer formulation.
FC experiment of HepG2 using 98753-3-RR (same clone as 98753-3-PBS)
1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Anti-Human VHL Rabbit RecAb (98753-3-RR, Clone: 251968A5) (red) or 0.25 ug Isotype Control (blue), and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00017). This data was developed using the same antibody clone with 98753-3-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Anti-Human VHL Rabbit RecAb (98753-3-RR, Clone: 251968A5) (red) or 0.25 ug Isotype Control (blue), and APC-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00017). This data was developed using the same antibody clone with 98753-3-PBS in a different storage buffer formulation.

Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

98753-3-PBS targets VHL in FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Eg6405 Product name: Recombinant human VHL protein Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 1-213 aa of NM_000551.4 Sequence: MPRRAENWDEAEVGAEEAGVEEYGPEEDGGEESGAEESGPEESGPEELGAEEEMEAGRPRPVLRSVNSREPSQVIFCNRSPRVVLPVWLNFDGEPQPYPTLPPGTGRRIHSYRGHLWLFRDAGTHDGLLVNQTELFVPSLNVDGQPIFANITLPVYTLKERCLQVVRSLVKPENYRRLDIVRSLYEDLEDHPNVQKDLERLTQERIAHQRMGD Predict reactive species
Full Name	von Hippel-Lindau tumor suppressor
Calculated Molecular Weight	24 kDa
GenBank Accession Number	NM_000551.4
Gene Symbol	VHL
Gene ID (NCBI)	7428
RRID	AB_3746321
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P40337
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

VHL (von Hippel-Lindau tumor suppressor) gene was identified as the tumor suppressor gene whose germ line mutations are associated with the inherited von Hippel-Lindau cancer syndrome (PMID: 8603073, 10722748). VHL patients develop a wide variety of tumors including retinal angioma, central nervous system hemangioblastoma, pheochromocytoma, and renal clear cell carcinoma (PMID: 10722748). VHL localizes predominantly to the cytoplasmic compartment but engages in a dynamic nuclear-cytoplasmic shuttle (PMID: 8700833, 9891082, 12101228).