VISTA Monoclonal antibody, PBS Only (Detector)

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  Sandwich ELISA standard curve of MP50058-1

  Sandwich ELISA standard curve of MP50058-1, VISTA Monoclonal Matched Antibody Pair - PBS only. 68676-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard  Eg0017. 68676-2-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.

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  Sample test of MP50058-1

  Serum of sixteen individual healthy human donors was measured. The VISTA concentration of detected samples was determined to be 525.25 pg/mL with a range of 52.46-1,435.23 pg/mL.

VISTA Monoclonal Antibody for Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 68676-2-PBS

Clone No.1D5C3

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

C10orf54, B7-H5, GI24, PP2135, SISP1

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Product Information

68676-2-PBS targets VISTA as part of a matched antibody pair:

MP50058-1: 68676-1-PBS capture and 68676-2-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Affinity:
  K_D=6.58 x 10^-11M
  K_Off=3.67 x 10^-6M
  K_On=5.58 x 10^4M
• Immunogen:

  CatNo: Eg0017

  Product name: Recombinant Human VISTA/C10orf54 protein (Myc Tag, His Tag)

  Source: mammalian cells-derived, pHZ-KIsec

  Tag: Myc & 6*His

  Domain: 33-194 aa of BC020568

  Sequence: FKVATPYSLYVCPEGQNVTLTCRLLGPVDKGHDVTFYKTWYRSSRGEVQTCSERRPIRNLTFQDLHLHHGGHQAANTSHDLAQRHGLESASDHHGNFSITMRNLTLLDSGLYCCLVVEIRHHHSEHRVHGAMELQVQTGKDAPSNCVVYPSSSQESENITAA

• Full Name: chromosome 10 open reading frame 54
• Calculated Molecular Weight: 311 aa, 34 kDa
• GenBank Accession Number: BC020568
• Gene Symbol: VISTA
• Gene ID (NCBI): 64115
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q9H7M9
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.