Tested Applications
| Positive FC detected in | human peripheral blood leukocytes |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 5 ul per 10^6 cells in 100 μl suspension |
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
APC-FcA98109 targets VISTA in FC applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg0017 Product name: Recombinant Human VISTA/C10orf54 protein (Myc Tag, His Tag) Source: mammalian cells-derived, pHZ-KIsec Tag: Myc & 6*His Domain: 33-194 aa of BC020568 Sequence: FKVATPYSLYVCPEGQNVTLTCRLLGPVDKGHDVTFYKTWYRSSRGEVQTCSERRPIRNLTFQDLHLHHGGHQAANTSHDLAQRHGLESASDHHGNFSITMRNLTLLDSGLYCCLVVEIRHHHSEHRVHGAMELQVQTGKDAPSNCVVYPSSSQESENITAA Predict reactive species |
| Full Name | chromosome 10 open reading frame 54 |
| Calculated Molecular Weight | 311 aa, 34 kDa |
| GenBank Accession Number | BC020568 |
| Gene Symbol | VISTA |
| Gene ID (NCBI) | 64115 |
| RRID | AB_3746529 |
| Conjugate | APC Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 650 nm / 660 nm |
| Excitation Laser | Red Laser (633 nm) |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9H7M9 |
| Storage Buffer | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
VISTA, also known as platelet receptor Gi24, B7-H5, C10orf54, Dies1, PD-1H, and SISP1, is a member of the immunoglobulin superfamily. It serves not only as a positive regulator but also as a substrate of MT1-MMP and contributes at least in part to tumor invasion.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for APC VISTA antibody APC-FcA98109 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
APC-FcA98109 is the FcZero-rAb® Anti-Human VISTA Rabbit Recombinant Antibody (clone 241003C8). It has 1 total citation. The cited publication appeared in International Immunopharmacology (Impact Factor 5.6), published in 2026. The research field is oncology and immunology, specifically investigating how ARID1B mutations promote immune evasion in acute myeloid leukemia through upregulation of VISTA expression.
| Species | Application | Title |
|---|---|---|
Int Immunopharmacol ARID1B mutations promote immune evasion in acute myeloid leukemia through upregulating VISTA expression. |
